Cold PSM, but not TRAIL, triggers autophagic cell death: A therapeutic advantage of PSM over TRAIL

International Journal of Oncology
Tomohisa ItoYoshihiro Suzuki-Karasaki

Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and cold plasma-stimulated medium (PSM) are promising novel anticancer tools due to their strong anticancer activities and high tumor-selectivity. The present study demonstrated that PSM and TRAIL may trigger autophagy in human malignant melanoma and osteosarcoma cells. Live-cell imaging revealed that even under nutritional and stress-free conditions, these cells possessed a substantial level of autophagosomes, which were localized in the cytoplasm separately from tubular mitochondria. In response to cytotoxic levels of PSM, the mitochondria became highly fragmented, and aggregated and colocalized with the autophagosomes. The cytotoxic effects of PSM were suppressed in response to various pharmacological autophagy inhibitors, including 3-methyladenine (3-MA) and bafilomycin A1, thus indicating the induction of autophagic cell death (ACD). Lethal levels of PSM also resulted in non-apoptotic, non-autophagic cell death in a reactive oxygen species-dependent manner under certain circumstances. Furthermore, TRAIL exhibited only a modest cytotoxicity toward these tumor cells, and did not induce ACD and mitochondrial aberration. The combined use of TRAIL and subtoxic conc...Continue Reading

References

Mar 26, 2003·Cell Death and Differentiation·H N LeBlanc, A Ashkenazi
Jun 6, 2003·Cytokine & Growth Factor Reviews·Alexandru Almasan, Avi Ashkenazi
Jun 6, 2003·Oncogene·Vladimir N IvanovZe'ev Ronai
Jan 10, 2004·Cell Death and Differentiation·T KanzawaS Kondo
Apr 13, 2004·Oncogene·Devrim Gozuacik, Adi Kimchi
Aug 25, 2004·The International Journal of Biochemistry & Cell Biology·Richard A Lockshin, Zahra Zakeri
Oct 26, 2005·Cell Death and Differentiation·P Codogno, A J Meijer
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
Oct 2, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Martin J S DyerGerald M Cohen
Aug 2, 2008·Autophagy·Sandra Díaz-TroyaJosé L Crespo
Sep 25, 2008·Nature Reviews. Cancer·Ricky W JohnstoneMark J Smyth
Mar 28, 2009·Cell Death and Differentiation·A Eisenberg-LernerA Kimchi
Jan 26, 2010·Nature Cell Biology·Sven GeislerWolfdieter Springer
Feb 4, 2010·PLoS Biology·Derek P NarendraRichard J Youle
Jun 10, 2010·Oncogene·F Gonzalvez, A Ashkenazi
Jan 18, 2011·The Journal of Biological Chemistry·Michael D DennisLeonard S Jefferson
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Oct 8, 2011·British Journal of Cancer·M KeidarB Trink
Jun 12, 2013·International Journal of Cancer. Journal International Du Cancer·Musarat IshaqKostya Ken Ostrikov
Jun 19, 2013·Advances in Cancer Research·Sujit K BhutiaPaul B Fisher
Jan 15, 2014·Apoptosis : an International Journal on Programmed Cell Death·Subhadip MukhopadhyaySujit Kumar Bhutia
Jul 7, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Koji ToriiYasuhiro Kodera
Feb 13, 2015·Future Oncology·Romain GuihoFrançoise Redini
Sep 10, 2015·International Journal of Oncology·Norifumi HattoriYasuhiro Kodera
Mar 5, 2016·Cell Death and Differentiation·D de MiguelL Martinez-Lostao
Jul 5, 2016·Frontiers in Pharmacology·Nestor Prieto-DomínguezJosé L Mauriz
Oct 26, 2016·International Journal of Oncology·Sung-Chul LimSong Iy Han
Feb 19, 2017·Biochemical and Biophysical Research Communications·Andrey V KulikovBoris Zhivotovsky
May 18, 2017·Frontiers in Oncology·Matteo BordiSilvia Campello
Jul 5, 2017·Frontiers in Oncology·Simone Fulda

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Citations

Mar 7, 2021·Journal of Clinical Medicine·Miguel Mateu-SanzCristina Canal

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BETA
protein assay
transfection

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H3A
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