Cold-Responsive Regions of Paradigm Cold-Shock and Non-Cold-Shock mRNAs Responsible for Cold Shock Translational Bias

International Journal of Molecular Sciences
Anna Maria GiuliodoriC O Gualerzi


In Escherichia coli, the mRNA transcribed from the main cold-shock gene cspA is a thermosensor, which at low temperature adopts a conformation particularly suitable for translation in the cold. Unlike cspA, its paralogue cspD is expressed only at 37 °C, is toxic so cannot be hyper-expressed in E. coli and is poorly translated in vitro, especially at low temperature. In this work, chimeric mRNAs consisting of different segments of cspA and cspD were constructed to determine if parts of cspA could confer cold-responsive properties to cspD to improve its expression. The activities of these chimeric mRNAs in translation and in partial steps of translation initiation such as formation of 30S initiation complexes and 50S subunits docking to 30S complexes to yield 70S initiation complexes were analyzed. We show that the 5' untranslated region (5'UTR) of cspA mRNA is sufficient to improve the translation of cspD mRNA at 37 °C whereas both the 5'UTR and the region immediately downstream the cspA mRNA initiation triplet are essential for translation at low temperature. Furthermore, the translational apparatus of cold-stressed cells contains trans-active elements targeting both 5'UTR and downstream regions of cspA mRNA, thereby improving ...Continue Reading


Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaC O Gualerzi
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J GoldsteinM Inouye
May 1, 1987·Journal of Bacteriology·P G JonesF C Neidhardt
Mar 1, 1994·Molecular Microbiology·P G Jones, M Inouye
Jun 1, 1994·Analytical Biochemistry·Marina V RodninaWolfgang Wintermeyer
Jan 1, 1996·Molecular Microbiology·A BrandiC L Pon
Apr 1, 1997·Molecular Microbiology·M L Sprengart, A G Porter
Apr 3, 1999·The Journal of Biological Chemistry·J P Etchegaray, M Inouye
Jul 20, 1999·Molecular Microbiology·J P Etchegaray, M Inouye
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·M O'ConnorA E Dahlberg
Jul 4, 2003·Science Progress·Michael H Weber, Mohamed A Marahiel
Aug 6, 2003·Journal of Molecular Biology·C O GualerziCynthia L Pon
Jan 20, 2004·RNA·Anna Maria GiuliodoriCynthia L Pon
Feb 6, 2004·Molecular Microbiology·Gianni ProssedaBianca Colonna
May 8, 2007·Annals of the New York Academy of Sciences·Anna Maria GiuliodoriMaría M Tavío
Oct 10, 2007·Methods in Enzymology·Letizia BrandiClaudio O Gualerzi
Jun 24, 2008·Molecular Cell·Pohl MilonMarina V Rodnina
Nov 17, 2009·Biochemical and Biophysical Research Communications·Younghoon Kim, Thomas K Wood
Jan 29, 2010·Environmental Microbiology·Younghoon KimThomas K Wood
Feb 5, 2010·Molecular Cell·Anna Maria GiuliodoriCynthia L Pon
Feb 11, 2014·Journal of Bacteriology·Sheetal UppalNarendra Jawali
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·C O Gualerzi, Cynthia L Pon
Mar 10, 2016·Journal of Molecular Biology·Lolita PiersimoniCynthia L Pon
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Attilio FabbrettiPaola Fucini
Nov 5, 2017·Biochemical Society Transactions·Erik Holmqvist, E Gerhart H Wagner


May 10, 2020·The Journal of Biological Chemistry·Alina GlaubZachary Ardern
Feb 16, 2021·Frontiers in Microbiology·He Cheng-Guang, C O Gualerzi

Related Concepts

Bacterial Proteins
Alkalescens-Dispar Group
Poly(A) Tail
Protein Biosynthesis
Gene Expression Regulation, Bacterial
5' Untranslated Regions
Cold-Shock Response
Escherichia coli
RNA, Messenger
Trans-Acting Responsive Region, HIV

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.