PMID: 7007814Jan 1, 1980Paper

Cold-sensitive mutations in beta and beta' subunit gene affecting the interaction of RNA polymerase with promoters

Molecular & General Genetics : MGG
A I GragerovV G. Nikiforov

Abstract

RNA polymerases with a cold-sensitive activity were purified from seven mutants of Escherichia coli. Subunit reconstitution experiments have shown that RNA polymerases from three mutants (Rpob262, RpoB264, and RpoB265) owed their cold sensitivity to alterations in the beta subunit. Three mutants (RpoC3, RpoC263, and RpoC267) were shown to be defective in the beta' subunit and one (RpoBC266) in both beta and beta' subunits. Two mutations (rpoC3 and rpoC263) reduced the level of RNA polymerase reconstitution. RNA polymerases from RpoC3 and RpoBC266 mutants are defective in RNA chain elongation at 6 degree C, while all the other mutants are defective in RNA polymerase-promoter interaction. most mutant RNA polymerases differ from the wild-type enzyme in transcription selectivity. The results obtained in this study indicate that both beta and beta' subunits are involved in RNA chain elongation and promoter binding and selection.

References

Jan 1, 1979·Annual Review of Genetics·T Yura, A Ishihama
Jan 1, 1979·Molecular & General Genetics : MGG·S M MirkinO A Larionov
Jun 6, 1977·Biochemical and Biophysical Research Communications·M SugiuraN Ito
Oct 20, 1977·Molecular & General Genetics : MGG·B S Young, A Wright
Sep 23, 1976·Molecular & General Genetics : MGG·G GrossE K Bautz
Apr 9, 1974·Molecular & General Genetics : MGG·V G NikiforovV V Velkov

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·A I GragerovV G Nikiforov
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·K Severinov, S A Darst
Jul 11, 1983·FEBS Letters·V G NikiforovO V Rokhlin
Jan 1, 1983·Molecular & General Genetics : MGG·H H Liebke, J F Speyer
Feb 1, 2000·The Journal of Biological Chemistry·K BrodolinV Nikiforov
Sep 1, 1982·Journal of Virology·L Fischer-Fantuzzi, C Vesco

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