Cold-storage preservation of the canine and rat pancreas prior to islet isolation

Transplantation
S R MunnD E Sutherland

Abstract

Cold-storage preservation of the canine pancreas prior to islet isolation has previously been noted to reduce the intrasplenic islet autograft success rate; but the mechanism of this deleterious effect has not been determined. We undertook a study in both outbred dogs and Lewis (RT1-1) rats to determine the influence of cold-storage preservation interval, preservation solution, and flushing technique on islet yield and islet viability. The preservation solutions used were those that had proved most efficacious in preserving segmental canine pancreases--namely, the modifications of silica gel fractionated plasma (SGF-III and SGF-IV) and an hydroxyethylstarch/lactobionate solution (UW-1). In the first set of experiments, the traditional vascular flush was used; this was followed by storage at 4 degrees C. After brief periods of preservation (3 hr in the rat, 12 hr in the dog) there was a significant (P less than 0.006) reduction in islet yield. The reduced yields were similar with each solution tested, were made worse with increasing intervals of storage, and resulted in a significant reduction in autograft success rate. The second set of experiments examined the effect of using an intraductal flush prior to preservation, along w...Continue Reading

Citations

Jun 7, 2008·Journal of Hepato-biliary-pancreatic Surgery·Hirofumi Noguchi, Shinichi Matsumoto
Dec 27, 2008·Journal of Hepato-biliary-pancreatic Surgery·Hirohito Ichii, Camillo Ricordi
Mar 17, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·S A WhiteM L Nicholson
Jan 9, 1998·Transplantation·Y TaniokaJ P Leone
Jul 17, 2004·Transplantation·Toshiaki TsujimuraJonathan R T Lakey
Jan 16, 2007·Transplantation·Tatsuya KinJonathan R T Lakey
Jan 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·K FederlinB J Hering
Nov 11, 2009·Transplant Immunology·M Reza MirbolookiJonathan R T Lakey
Jun 15, 2004·Transplantation Proceedings·E J WoodsJ K Critser
May 1, 1989·The Journal of Surgical Research·M J FieldS R Munn
Feb 7, 2012·Cell Transplantation·Anthony R HoganAntonello Pileggi
Dec 17, 2010·World Journal of Gastrointestinal Surgery·Hirofumi Noguchi
Mar 1, 2012·World Journal of Gastrointestinal Pathophysiology·Naoaki SakataMichiaki Unno
Dec 24, 2011·World Journal of Transplantation·Takashi Kuise, Hirofumi Noguchi
Mar 1, 1990·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·I H al-AbdullahG M Abouna

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.