Cold stratification, temperature, light, GA3 , and KNO3 effects on seed germination of Primula beesiana from Yunnan, China

Plant Diversity
Li-E YangHang Sun

Abstract

Primula beesiana Forr. is an attractive wildflower endemically distributed in the wet habitats of subalpine/alpine regions of southwestern China. This study is an attempt to understand how this plant adapts to wet habitats and high altitudes. Specifically, we examined the effects of cold stratification, light, GA3, KNO3, and temperature on P. beesiana seed germination. KNO3 and GA3 increased germination percentage and germination rate compared to control treatments at 15/5 and 25/15 °C. Untreated seeds germinated well (> 80%) at higher temperatures (20, 25 and 28 °C), whereas at lower (5, 10 and 15 °C) and extremely high temperatures (30 and 32 °C) germination decreased significantly. However, after cold stratification (4-16 weeks), the germination percentage of P. beesiana seeds at low temperatures (5-15 °C) and the germination rate at high temperatures (30 °C) increased significantly, suggesting that P. beesiana has type 3 non-deep physiological dormancy. The base temperature and thermal time for germination decreased in seeds that were cold stratified for 16 weeks. Cold-stratified seeds incubated at fluctuating temperatures (especially at 15/5 °C) had significantly high germination percentages and germination rates in light,...Continue Reading

Software Mentioned

PASW Statistics
OriginPro

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