Cold-tolerant introgression line construction and low-temperature stress response analysis for bell pepper.

Plant Signaling & Behavior
Lusha JiShangjing Guo

Abstract

We aimed to screen cold-tolerant introgression lines (ILs) of bell pepper and investigate stress responses of these bell peppers under low temperature. Seedlings of cold-resistant wild-type bell pepper CA157, cultivated bell pepper CA52, and their ILs were evaluated for their tolerance to low temperature. Electrical conductivity measurement was performed on ILs and two parents. Then, contents of physiological and biochemical indexes including malondialdehyde (MDA), proline, and soluble sugar content were examined. Moreover, the superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD) activities were further investigated. Finally, the chlorophyll fluorescence (PSII) parameters in all pant leaves were examined. A total of 22 IL plants showed better resistance than their recurrent parent CA52. CL122 was one of the most outstanding plants in ILs that had similar performance with wild bell pepper CA157. Cold resistance analysis based on physiological and biochemical indexes showed that factors such as electrical conductivity, MDA, and PSII were closely related to cold resistance among CA157, CA52, and CL122 under low-temperature stress. In conclusion, ILs constructed in the current study might be ...Continue Reading

References

May 17, 2003·Journal of Plant Physiology·Paula Scotti CamposMaria Antonieta Nunes
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Aug 21, 2013·Plant Physiology·Katherine R M MackeyMak A Saito
Nov 5, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P M EgginkR G F Visser
Feb 22, 2014·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yaghoub SafdariHossein Zarei Jaliani
Mar 1, 2004·The New Phytologist·Astrid WinglerNathalie Pourtau

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Methods Mentioned

BETA
environmental stress
transgenic
MDA

Software Mentioned

SigmaPlot
Systat

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