PMID: 6968799Jan 1, 1980Paper

Colicin E1 plasmid as a probe for detection and study of anti-dna activity in SLE sera

Journal of Immunological Methods
G F Casperson, E W Voss

Abstract

Tritiated colicin E1 plasmid DNA ([3H]DNA) was purified, characterized and developed as a test antigen for study of anti-DNA antibody activity in a modified Farr assay. The homogeneous molecular weight (4.2 X 10(6) daltons), physical state (intact covalently closed circles), and capacity for intrinsic radioactive labeling to a suitable specific activity were all important properties of the plasmid probe. In addition, the ability of the Farr assay to measure primary antigen/antibody interactions made quantitative determinations of anti-double-stranded DNA (dsDNA) antibody activity possible. Sonication of the plasmid resulted in definable fragments which were thermally denatured and used in the measurement of anti-single-stranded DNA (ssDNA) antibody activity. Results from studies in which unlabeled dsDNA, ssDNA, amd free 2'-deoxyribonucleotides were employed to inhibit binding of [3H]DNA by anti-DNA antibodies indicated the presence of 3 distinct anti-DNA specificities in SLE sera.

References

Feb 1, 1977·Annals of the Rheumatic Diseases·H R Schumacher
Jan 1, 1979·Journal of Immunological Methods·J L KlotzR L Teplitz
Jan 1, 1976·Journal of Immunological Methods·L A AardenE R De Groot
Jan 1, 1976·Journal of Immunological Methods·L A ArdenT E Feltkamp
Oct 1, 1975·Nucleic Acids Research·T M ShinnickF R Blattner
Mar 25, 1976·Journal of Molecular Biology·R J RobertsK Murray
Oct 1, 1967·The Journal of Experimental Medicine·D KofflerH G Kunkel
Oct 1, 1973·Analytical Biochemistry·W L HughesD Stollar
Aug 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R T EspejoR L Sinsheimer
Apr 1, 1968·Virology·R T Espejo, E S Canelo
Sep 25, 1969·The New England Journal of Medicine·T PincusN Talal
Nov 1, 1967·The Journal of Clinical Investigation·R Arana, M Seligmann
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·O J PLESCIAN C PALCZUK

❮ Previous
Next ❯

Citations

Mar 10, 1989·Journal of Immunological Methods·Y N Vaishnav, A Antony
Jun 1, 1982·Molecular Immunology·D W Ballard, E W Voss
Apr 1, 1982·Molecular Immunology·M R JarvisE W Voss
Jun 1, 1987·Annals of the Rheumatic Diseases·D A IsenbergA Todd-Pokropek
Aug 1, 1990·Immunological Investigations·Y N Vaishnav, A Antony

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.