Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains

Scientific Reports
Lenka MicenkováDavid Smajs

Abstract

Colicin production in Escherichia coli (E. coli) strains represents an important trait with regard to microbial survival and competition in the complex intestinal environment. A novel colicin type, colicin Z (26.3 kDa), was described as a product of an original producer, extraintestinal E. coli B1356 strain, isolated from the anorectal abscess of a 17 years-old man. The 4,007 bp plasmid (pColZ) was completely sequenced and colicin Z activity (cza) and colicin Z immunity (czi) genes were identified. The cza and czi genes are transcribed in opposite directions and encode for 237 and 151 amino acid-long proteins, respectively. Colicin Z shows a narrow inhibitory spectrum, being active only against enteroinvasive E. coli (EIEC) and Shigella strains via CjrC receptor recognition and CjrB- and ExbB-, ExbD-mediated colicin translocation. All tested EIEC and Shigella strains isolated between the years 1958-2010 were sensitive to colicin Z. The lethal effect of colicin Z was found to be directed against cell wall peptidoglycan (PG) resulting in PG degradation, as revealed by experiments with Remazol Brilliant Blue-stained purified peptidoglycans and with MALDI-TOF MS analyses of treated PG. Colicin Z represents a new class of colicins t...Continue Reading

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Citations

Aug 29, 2020·Expert Review of Anti-infective Therapy·Juraj BosákDavid Šmajs
May 4, 2021·Oxidative Medicine and Cellular Longevity·Fuqing HuangJin Zhong
Sep 22, 2021·The FEBS Journal·Stephanie Sibinelli-SousaEthel Bayer-Santos

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Datasets Mentioned

BETA
MK5999282

Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

SignalP
DNASTAR
ORF finder
Molecular Evolutionary Genetics Analysis ( MEGA )
BLAST
PSORTb
DNASTAR Lasergene
ExPASy
TASSER
TMPred

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