Colistin causes profound morphological alteration but minimal cytoplasmic membrane perforation in populations of Escherichia coli and Pseudomonas aeruginosa

Archives of Microbiology
Noelle H O'DriscollAndrew J Lamb

Abstract

Whilst colistin (polymyxin E) represents the last mainstream treatment option for multidrug-resistant Gram-negative pathogens, details of its mechanism of action remain to be fully resolved. In this study, the effects of sub-inhibitory, inhibitory-bactericidal, and supra-bactericidal levels of colistin on the membrane integrity and morphology of Escherichia coli and Pseudomonas aeruginosa were investigated using potassium loss, flow cytometry, and scanning electron microscopy (SEM). Supra-bactericidal colistin concentrations induced just 4-12% intracellular potassium loss from bacteria after 24 h. Flow cytometry data suggested colistin might alter cell arrangement, and SEM confirmed the antibiotic causes bacterial aggregation. Filamentation was not detected in either species at any concentration or time-point up to 24 h. These results argue against the hypotheses that colistin kills bacteria by puncturing the cytoplasmic membrane or disrupting DNA synthesis. The colistin-induced bacterial aggregation detected has implications for the interpretation of MBC, time-kill, and other test results obtained with this antibiotic.

References

Nov 1, 1986·The Journal of Antimicrobial Chemotherapy·J M Diver, R Wise
Jan 1, 1969·Journal of Bacteriology·M KoikeT Matsuo
May 21, 1999·Science·J W CostertonE P Greenberg
Mar 1, 2002·Journal of Microbiological Methods·Dmitri S OrlovRobert I Lehrer
Aug 26, 2006·The Lancet Infectious Diseases·Jian LiDavid L Paterson
Nov 6, 2007·Current Opinion in Microbiology·Kevin D Young
Nov 6, 2007·Current Opinion in Microbiology·Mary J Osborn, Lawrence Rothfield
Nov 29, 2007·Pflügers Archiv : European journal of physiology·David AlsteensYves F Dufrêne
Dec 23, 2008·Chemosphere·T P Tim CushnieJeanette M C Robertson
Feb 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Ninell P MortensenMitchel J Doktycz
Oct 31, 2009·Journal of Medicinal Chemistry·Tony VelkovJian Li
Nov 26, 2009·Peptides·Dan BolintineanuYiannis N Kaznessis
Jun 17, 2010·The Journal of Antimicrobial Chemotherapy·Kamel AlhanoutJean Michel Brunel
Nov 1, 2011·Applied and Environmental Microbiology·Brandon W PetersonHenk J Busscher
Apr 24, 2013·Molecular BioSystems·Kelly C PeachRoger G Linington
May 25, 2013·Future Microbiology·Tony VelkovJian Li
Mar 8, 2014·Critical Care : the Official Journal of the Critical Care Forum·Patrick M HonoréHerbert D Spapen
Mar 19, 2014·The Journal of Antimicrobial Chemotherapy·Ursula Theuretzbacher
Feb 3, 2015·Seminars in Respiratory and Critical Care Medicine·Cornelia B Landersdorfer, Roger L Nation
Jun 2, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C G Giske
Sep 15, 2015·Expert Opinion on Drug Safety·Theodoros Kelesidis, Matthew E Falagas
Dec 18, 2015·Colloids and Surfaces. B, Biointerfaces·Jialiang LiangMeiping Tong
Jul 10, 2016·Cellular and Molecular Life Sciences : CMLS·T P Tim CushnieAndrew J Lamb
Aug 16, 2016·International Journal of Antimicrobial Agents·Sophie BaronAbiola Olumuyiwa Olaitan
Oct 8, 2016·Postgraduate Medicine·Mazen S BaderAnnie A Brooks
Oct 30, 2016·International Journal of Antimicrobial Agents·Thien B TranJian Li
Mar 23, 2017·BioMed Research International·Zhiliang YuJuanping Qiu

Citations

May 18, 2021·Frontiers in Microbiology·Anastasia Cianciulli SessoUdo Bläsi

Methods Mentioned

BETA
transmission electron microscopy
flow
scanning electron microscopy
flow cytometry
atomic force microscopy

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