Collaboration between CC- and A-adding enzymes to build and repair the 3'-terminal CCA of tRNA in Aquifex aeolicus

Science
Kozo Tomita, A M Weiner

Abstract

The universal 3'-terminal CCA sequence of all transfer RNAs (tRNAs) is repaired, and sometimes constructed de novo, by the CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase]. This RNA polymerase has no nucleic acid template, yet faithfully builds the CCA sequence one nucleotide at a time using cytidine triphosphate (CTP) and adenosine triphosphate (ATP) as substrates. All previously characterized CCA-adding enzymes from all three kingdoms are single polypeptides with CCA-adding activity. Here, we demonstrate through biochemical and genetic approaches that CCA addition in Aquifex aeolicus requires collaboration between two related polypeptides, one that adds CC and another that adds A.

Citations

Oct 3, 2002·Biochemical and Biophysical Research Communications·Masaki GoudaKazuya Nishikawa
Nov 30, 2002·Biochimie·Dagmar K WillkommRoland K Hartmann
Aug 6, 2004·Nature·Kozo TomitaOsamu Nureki
Aug 28, 2004·Nature Structural & Molecular Biology·Paul Schimmel, Xiang-Lei Yang
Jun 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anne NeuenfeldtMario Mörl
Dec 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sandy TretbarMario Mörl
Mar 30, 2010·Nucleic Acids Research·Andrea HoffmeierMario Mörl
Jul 22, 2011·Nucleic Acids Research·Xiao-Long ZhouEn-Duo Wang
Dec 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hyundae D ChoAlan M Weiner
Feb 16, 2010·Cellular and Molecular Life Sciences : CMLS·Heike BetatMario Mörl
Nov 4, 2009·FEBS Letters·Stefan Vörtler, Mario Mörl
Jul 15, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Heike Betat, Mario Mörl
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Jan 16, 2020·International Journal of Molecular Sciences·Lieselotte ErberMario Mörl
Nov 30, 2018·Frontiers in Genetics·Yuka Yashiro, Kozo Tomita
Jul 29, 2020·International Journal of Molecular Sciences·Lieselotte ErberMario Mörl

Related Concepts

CCA-adding enzyme, human
CC adding enzyme
A adding enzyme
Adenosine Triphosphate, Chromium Ammonium Salt
Mg CTP
Gram-Negative Bacteria
Proteins, Recombinant DNA
RNA Nucleotidyltransferases
RNA, Bacterial
Transfer RNA

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