Collaborative study on fifteen compounds in the rat-liver Comet assay integrated into 2- and 4-week repeat-dose studies

Mutation Research
Andreas RothfussSheila Galloway

Abstract

A collaborative trial was conducted to evaluate the possibility of integrating the rat-liver Comet assay into repeat-dose toxicity studies. Fourteen laboratories from Europe, Japan and the USA tested fifteen chemicals. Two chemicals had been previously shown to induce micronuclei in an acute protocol, but were found negative in a 4-week Micronucleus (MN) Assay (benzo[a]pyrene and 1,2-dimethylhydrazine; Hamada et al., 2001); four genotoxic rat-liver carcinogens that were negative in the MN assay in bone marrow or blood (2,6-dinitrotoluene, dimethylnitrosamine, 1,2-dibromomethane, and 2-amino-3-methylimidazo[4,5-f]quinoline); three compounds used in the ongoing JaCVAM (Japanese Center for the Validation of Alternative Methods) validation study of the acute liver Comet assay (2,4-diaminotoluene, 2,6-diaminotoluene and acrylamide); three pharmaceutical-like compounds (chlordiazepoxide, pyrimethamine and gemifloxacin), and three non-genotoxic rodent liver carcinogens (methapyrilene, clofibrate and phenobarbital). Male rats received oral administrations of the test compounds, daily for two or four weeks. The top dose was meant to be the highest dose producing clinical signs or histopathological effects without causing mortality, i.e....Continue Reading

References

Feb 1, 1992·Mutation Research·A O AsitaT Sofuni
Jan 1, 1992·Environmental and Molecular Mutagenesis·B E ButterworthM E Hurtt
Jan 1, 1989·Environmental and Molecular Mutagenesis·Y SuzukiH Shimizu
Jan 1, 1995·Environmental and Molecular Mutagenesis·G Krishna, J C Theiss
Mar 1, 1995·Mutation Research·M L GarriottL S Schwier
Jun 29, 1999·Carcinogenesis·B S ShaneM L Cunningham
Sep 9, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·R M DavidD Guest
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
May 30, 2002·Mutation Research·Kaoru SekihashiYu F Sasaki
Dec 11, 2002·Mutagenesis·A HartmannUNKNOWN 4th International Comet Assay Workshop
Mar 7, 2003·Mutagenesis·Stig Johan Wiklund, Eva Agurell
Jan 26, 2005·Mutation Research·Isabelle ManièreJean-Michel Poul
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Apr 4, 2008·Mutagenesis·David P Lovell, Takashi Omori
Oct 11, 2008·Mutation Research·Giovanni Brambilla, Antonietta Martelli
Jun 9, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y Yener, M Dikmenli

❮ Previous
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Citations

Aug 28, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephen D DertingerJames T Macgregor
Nov 10, 2011·Toxins·Nicole SchuppHelga Stopper
Oct 26, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Nikolai P SirotaAndrei D Durnev
Jan 14, 2012·Mutation Research·Tomoyasu ToyoizumiKohji Yamakage
Oct 22, 2011·Toxicology·Catherine C PriestleyDominic P Williams
Dec 22, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Vasily N DobrovolskyWei Ding
Aug 8, 2015·Environmental and Molecular Mutagenesis·Ulla Plappert-Helbig, Melanie Guérard
Dec 1, 2011·Pharmaceutical Statistics·Jonathan BrightRichardus Vonk
Nov 5, 2011·Environmental and Molecular Mutagenesis·Javed A BhalliHans-Werner Vohr
Jul 1, 2014·Environmental and Molecular Mutagenesis·Melissa R SchislerDarrell R Boverhof
Dec 19, 2013·Environmental and Molecular Mutagenesis·M GuérardU Plappert-Helbig
May 14, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Takeshi MoritaMasamitsu Honma
Mar 7, 2014·Environmental and Molecular Mutagenesis·William C GuntherKrista L Dobo
Apr 20, 2016·Frontiers in Microbiology·Jaewoo BaiJu-Hoon Lee
Jul 28, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Cheryl A HobbsKristine L Witt
Aug 2, 2011·The Journal of Toxicological Sciences·Hiroshi SuzukiShin-ichi Sato
May 9, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Günter SpeitDan D Levy
May 9, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Yoshifumi UnoHironao Takasawa
May 29, 2012·Mutation Research·Marie Z Vasquez
Apr 22, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Tadashi ImamuraShin-ichi Sato
Apr 29, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Kenji InoueTakaaki Doi
Dec 2, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Bas-Jan van der LeedeVéronique Thybaud
Apr 22, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Tomomi TakayanagiWataru Fujii
May 25, 2012·Carcinogenesis·C MagkoufopoulouJ H M van Delft
Dec 7, 2011·Critical Reviews in Toxicology·Tatyana Y DoktorovaVera Rogiers
Mar 7, 2013·Mutagenesis·Kristine B GutzkowGunnar Brunborg
Sep 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tao WangWilliam David McGuinn
Jun 11, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wei DingMugimane G Manjanatha
Jul 31, 2012·Mutagenesis·Lykke ForchhammerPeter Møller
Aug 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Raffaella Corvi, Federica Madia
Oct 12, 2018·Environmental and Molecular Mutagenesis·Ulla Plappert-HelbigHans-Jörg Martus

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