Collagen gene expression and mechanical properties of intervertebral disc cell-alginate cultures

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
A E BaerLori A Setton

Abstract

Cells of the intervertebral disc have a limited capacity for matrix repair that may contribute to the onset and progression of degenerative disc changes. In this study, the biosynthetic capacity of cells isolated from specific regions of the porcine intervertebral disc was evaluated in vitro. Using a competitive reverse transcription-polymerase chain reaction technique, gene expression levels for types I and II collagen were quantified in cells cultured for up to 21 d in a three-dimensional alginate culture system and compared to levels obtained for cells in vivo. The mechanical properties of cell-alginate constructs were measured in compression and shear after periods of culture up to 16 weeks. Cells from the anulus fibrosus expressed the most type I collagen mRNA in vivo and in vitro, while cells from the transition zone expressed the most type II collagen mRNA in vivo and in vitro. Mechanical testing results indicate that a mechanically functional matrix did not form at any time during the culture period; rather, decreases of up to 50% were observed in the compressive and shear moduli of the cell-alginate constructs compared to alginate with no cells. Together with results of prior studies, these results suggest that interve...Continue Reading

References

Sep 11, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B A Maldonado, T R Oegema
Nov 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M D BuschmannE B Hunziker
Apr 1, 1992·Matrix : Collagen and Related Research·H J HäuselmannE J Thonar
Mar 1, 1991·Spine·J P ThompsonD S Bradford
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Nov 1, 1989·The Journal of Cell Biology·P BrucknerM A Spycher
Jan 1, 1986·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M T BaylissS Holm
Jul 1, 1995·FEMS Microbiology Reviews·I W Sutherland
Jul 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A SettonD S Howell
Jan 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OhshimaD H Bergel
May 1, 1997·The American Journal of Physiology·H IshiharaJ P Urban
Dec 10, 1997·Journal of Biomechanics·J C IatridisV C Mow
May 20, 1998·Arthritis and Rheumatism·J L HuebnerV B Kraus
Jan 12, 1999·Experimental Cell Research·D J AguiarT R Oegema
Jul 10, 1999·Journal of Biomedical Materials Research·M A LeRouxL A Setton
Nov 24, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J C IatridisV C Mow
Jan 13, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H Ishihara, J P Urban
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K MasudaE Thonar

❮ Previous
Next ❯

Citations

Apr 21, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·G ChangR A Kandel
Mar 5, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Jun ChenLori A Setton
May 24, 2007·Tissue Engineering·Damien M O'Halloran, Abhay S Pandit
May 18, 2011·Tissue Engineering. Part C, Methods·Diana Ribeiro PereiraRui L Reis
Jul 1, 2005·Arthritis Research & Therapy·Christine Lyn Le MaitreJudith Alison Hoyland
Dec 18, 2013·Acta Biomaterialia·Sílvia J BidarraPedro L Granja
Oct 10, 2013·Tissue Engineering. Part a·Hai WangCheng-Jie Xiong
Apr 25, 2007·Connective Tissue Research·Michitsuna KatafuchiMitsuo Yamauchi
Sep 2, 2014·Advanced Drug Delivery Reviews·Gianluca FontanaAbhay Pandit
Sep 25, 2012·Acta Biomaterialia·Lucia K Y ChanKenneth M C Cheung
Jan 19, 2010·Journal of Biomechanics·Nandan L NerurkarRobert L Mauck
Oct 26, 2007·Biomaterials·Damien O HalloranAbhay S Pandit
Dec 6, 2005·Neurosurgery Clinics of North America·J C Lotz, A J Kim
Sep 13, 2008·Journal of Biomedical Materials Research. Part a·Alice I Chou, Steven B Nicoll
Dec 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Casey L KoreckiJames C Iatridis
Nov 11, 2011·Journal of Tissue Engineering and Regenerative Medicine·D R PereiraR L Reis
Jul 29, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mostafa A GabrJun Chen
Jun 6, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D T BridgenL A Setton
Jan 24, 2009·Journal of Biomedical Materials Research. Part a·Godfrey ChangRita Kandel
Oct 6, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Bo HuangChuan-Zhi Zhang
Aug 13, 2005·Journal of Biomechanics·James E GuilkeyJeffrey A Weiss
May 28, 2009·Journal of Cellular Physiology·Anshu RastogiAdam H Hsieh
Mar 21, 2013·Journal of Tissue Engineering and Regenerative Medicine·Gianluca VadalàVincenzo Denaro
Nov 22, 2011·Journal of the Mechanical Behavior of Biomedical Materials·Tristan P DriscollRobert L Mauck
Aug 6, 2013·Biotechnology Advances·Joana Silva-CorreiaRui L Reis
Nov 20, 2014·Cell and Tissue Research·Silvia GonzalesChun-Yuh Charles Huang
Mar 29, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xinyan TangJun Chen
Jun 5, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Daniel G T Strange, Michelle L Oyen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.