Collagen-induced arthritis

Current Protocols in Immunology
Edward F RosloniecLinda K Myers

Abstract

Collagen-induced arthritis (CIA) is an experimental autoimmune disease that can be elicited in susceptible strains of rodents (rat and mouse) and nonhuman primates by immunization with type II collagen (CII), the major constituent protein of articular cartilage. Because of the important similarities between CIA and rheumatoid arthritis, this experimental model of autoimmune arthritis has been the subject of extensive investigation in several laboratories. Protocols for CIA are described in this unit for both the mouse model and the rat model. In addition, protocols are included for the purification of CII from bovine articular joints and chicken sternums, for the purification of collagen a1(II) chains, and for the purification of fragments of these chains following cyanogen bromide (CNBr) digestion. The preparation of CII is a time-consuming procedure but is usually required because of the scarcity and expense of commercial sources of purified native CII. In addition, support protocols are provided for assessing the severity of inflammation following CIA and for measuring B and T cell responses to CII.

References

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Citations

Dec 2, 2015·Experimental and Therapeutic Medicine·Hou-Pan SongXiong Cai
Jul 18, 2014·BioMed Research International·Sayuri YoshimuraAkio Nakane
May 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhaohui QianEdward F Rosloniec
Nov 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeoung-Eun ParkLinda K Myers
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda K MyersAndrew H Kang
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa E Munroe, Gail A Bishop
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael A CremerAndrew H Kang
Jun 7, 2017·Inflammation·Zun-Zhong LiQing-Hua Li
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jin-Sil ParkYoung-Woo Park
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·David D BrandEdward F Rosloniec

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