Collagen lattice effects on fibroblast arachidonic acid metabolism

Journal of Cellular Physiology
A P Pentland

Abstract

Fibroblasts are routinely maintained in vitro on tissue culture plastic, in an environment which is devoid of collagen, the most abundant extracellular protein in dermis. Recent work has shown that by seeding fibroblasts into a collagen matrix, many aspects of their metabolism change dramatically: they stop proliferation, organize and contract the collagen matrix, and secrete much larger quantities of the usual extracellular matrix components. Because so many fibroblast functions are dramatically altered by the presence of the collagen matrix, matrix effects on fibroblast metabolism of arachidonic acid were examined. The studies presented here show that during the period of matrix contraction, metabolism of arachidonate to prostaglandins by fibroblasts is increased sixfold compared to cells plated on plastic, and that this increase is correlated with contraction but does not regulate it. The increase in prostaglandin synthesis is due in part to an increased new synthesis of the rate-limiting enzyme in prostaglandin synthesis, cyclooxygenase. No change in the profile of products the fibroblasts synthesize from arachidonate is induced by the presence of the matrix. After the lattice contraction is complete, the basal arachidonate...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Jan 1, 1985·Annual Review of Immunology·S K DurumJ J Oppenheim
Jun 1, 1987·The Journal of Investigative Dermatology·A Z EisenG I Goldberg
Jan 1, 1986·The Journal of Clinical Investigation·A P Pentland, P Needleman
Jan 3, 1980·Biochimica Et Biophysica Acta·D SomjenA Harell
Jul 1, 1983·The Journal of Investigative Dermatology·E BellY Neveux
Dec 1, 1984·The Journal of Clinical Investigation·P J Whiteley, P Needleman
Nov 1, 1981·Prostaglandins·L Taylor, P Polgar

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Citations

Oct 29, 2003·Tissue & Cell·S CarnevaliS I Rennard
Jun 3, 1999·Proceedings of the Association of American Physicians·C M SköldS I Rennard
Jul 1, 1994·The Journal of Cell Biology·Y He, F Grinnell
May 12, 1998·The American Journal of Physiology·S CarnevaliS I Rennard
Sep 23, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M SköldS I Rennard
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hui Jung KimStephen I Rennard

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