Collagen matrix as a tool in studying fibroblastic cell behavior

Cell Adhesion & Migration
Jiří Kanta

Abstract

Type I collagen is a fibrillar protein, a member of a large family of collagen proteins. It is present in most body tissues, usually in combination with other collagens and other components of extracellular matrix. Its synthesis is increased in various pathological situations, in healing wounds, in fibrotic tissues and in many tumors. After extraction from collagen-rich tissues it is widely used in studies of cell behavior, especially those of fibroblasts and myofibroblasts. Cells cultured in a classical way, on planar plastic dishes, lack the third dimension that is characteristic of body tissues. Collagen I forms gel at neutral pH and may become a basis of a 3D matrix that better mimics conditions in tissue than plastic dishes.

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Dec 1, 1989·The Journal of Investigative Dermatology·S NakagawaF Grinnell
Nov 1, 1986·Experimental Cell Research·P GilleryJ P Borel
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Jun 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·R S Bienkowski, M G Gotkin
Mar 1, 1995·Experimental Cell Research·N L Halliday, J J Tomasek
Feb 1, 1994·The Journal of Cell Biology·F Grinnell
Apr 1, 1994·Journal of Cellular Physiology·P D Arora, C A McCulloch
Feb 11, 1998·The Journal of Investigative Dermatology·J FluckS Sollberg
Apr 3, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·M IvarssonK Rubin
May 5, 1998·Molecular and Cellular Biology·H RosenfeldtF Grinnell
Mar 6, 1999·Experimental Cell Research·J TrächslinM Chiquet
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N ThéretB Clément
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M PréauxP Mavier
Oct 28, 1999·The Journal of Investigative Dermatology. Symposium Proceedings·K YoshizatoY Morpho
Feb 1, 2000·The Journal of Biological Chemistry·H Rosenfeldt, F Grinnell
Mar 25, 2000·The Journal of Investigative Dermatology·G D PinsJ R Morgan
Apr 1, 2000·The Journal of Biological Chemistry·D C HockingK J Langenbach
Jul 8, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·R T PrajapatiR A Brown
Sep 30, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M VarediP G Scott
May 12, 2001·The Journal of Investigative Dermatology·S NilandS Sollberg
Jul 20, 2001·Annals of the New York Academy of Sciences·R A Clark

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Citations

Mar 2, 2016·Current Opinion in Biotechnology·Ana M PorrasKristyn S Masters
Feb 9, 2018·Photochemistry and Photobiology·Wongnapa NakyaiCéline Viennet
Jan 19, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gerald BurgstallerOliver Eickelberg
Aug 15, 2018·Advanced Healthcare Materials·Hyun Jong LeeDavid Myung
Oct 16, 2018·Tissue Engineering. Part a·Sweelin ChewKeng-Hwee Chiam
Jan 25, 2020·Biofabrication·Cheng-Kuang HuangKeng-Hui Lin
Nov 4, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anjian XuJian Huang
Aug 19, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ana Luísa CartaxoCatarina Brito
Sep 14, 2018·International Journal of Molecular Sciences·Kentaro Hozumi, Motoyoshi Nomizu
Jun 27, 2020·In Vitro Cellular & Developmental Biology. Animal·Yuko Hara-SaitoKenji Izumi
Jul 25, 2020·Tissue Engineering. Part a·Yuanjia ZhuYi-Ping Joseph Woo
Jun 26, 2020·BioMed Research International·Francesca LombardiBenedetta Cinque
Aug 29, 2021·Polymers·Ruth NaomiHasnah Bahari
Oct 12, 2021·ACS Applied Materials & Interfaces·Venkanagouda S GoudarFan-Gang Tseng
Nov 10, 2021·Biomacromolecules·Michèle DaiSébastien Lecommandoux

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Methods Mentioned

BETA
GTPase
GTPases

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