Collagen scaffold enhances the regenerative properties of mesenchymal stromal cells

PloS One
Iran RashediArmand Keating

Abstract

MSCs are widely applied to regenerate heart tissue in myocardial diseases but when grown in standard two-dimensional (2D) cultures exhibit limited potential for cardiac repair and develop fibrogenic features with increasing culture time. MSCs can undergo partial cardiomyogenic differentiation, which improves their cardiac repair capacity. When applied to collagen patches they may improve cardiac tissue regeneration but the mechanisms remain elusive. Here, we investigated the regenerative properties of MSCs grown in a collagen scaffold as a three-dimensional (3D) culture system, and performed functional analysis using an engineered heart tissue (EHT) model. We showed that the expression of cardiomyocyte-specific proteins by MSCs co-cultured with rat neonatal cardiomyocytes was increased in collagen patches versus conventional cultures. MSCs in 3D collagen patches were less fibrogenic, secreted more cardiotrophic factors, retained anti-apoptotic and immunomodulatory function, and responded less to TLR4 ligand lipopolysaccharide (LPS) stimulation. EHT analysis showed no effects by MSCs on cardiomyocyte function, whereas control dermal fibroblasts abrogated the beating of cardiac tissue constructs. We conclude that 3D collagen scaf...Continue Reading

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Aug 21, 2019·Journal of Cardiovascular Development and Disease·Michael P Czubryt
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Methods Mentioned

BETA
flow cytometry
magnetic activated cell sorting
flow cytometery
fluorescence-activated cell sorting
PCR
scraping
Protein assay
ELISA
antibody array
FACS

Software Mentioned

Prism4
GraphPad
ImageJ
FlowJo
NIH
Image
Fiji

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