Collagen sponge scaffolds containing growth factors for the functional regeneration of tracheal epithelium

Journal of Tissue Engineering and Regenerative Medicine
Koichi OmoriKoichi Omori

Abstract

Tracheal epithelia have barrier and mucociliary clearance functions that prevent invasion of extraneous particles and infectious materials. Hence, following tracheal reconstructions, functional and morphological regeneration of epithelia is required to prevent respiratory declines and infectious diseases. Although growth factors (GFs) promote the regeneration of tracheal epithelial morphologies, it remains unclear whether tracheal grafts containing GFs are beneficial for regeneration of tracheal epithelial functions. Thus, we fabricated collagen sponge scaffolds containing insulin-like GF-1 (IGF-1) and the basic fibroblast, hepatocyte, and epidermal GFs (bFGFs, HGFs, and EGFs, respectively), and we evaluated the effects of the grafts on the functional regeneration of tracheal epithelia. Partial tracheal defects were imposed surgically, and collagen sponges containing IGF-1, bFGF, HGF, or EGF were then transplanted to defect sites. Subsequent immunofluorescence studies suggested that EGF and bFGF contribute to regular distributions of tight junction molecules, and tracer permeability assays suggested that EGF and bFGF promote regeneration of barrier function. Increased ciliogenesis was also observed using scanning electron micro...Continue Reading

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Citations

Sep 6, 2020·Interactive Cardiovascular and Thoracic Surgery·Yuichiro UedaHiroshi Date
Feb 18, 2020·Journal of Tissue Engineering and Regenerative Medicine·Archna DhasmanaSagar Rawal
Feb 11, 2020·Frontiers in Bioengineering and Biotechnology·Xiaochen RenZiad Julier
Nov 18, 2020·Research in Veterinary Science·Haitham G Abo-Al-Ela
May 20, 2021·Journal of Tissue Engineering and Regenerative Medicine·Ryosuke NakamuraKoichi Omori
Jan 22, 2022·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Cai-Sheng LiuMin-Qiao Jian

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