Collagen Stiffness and Architecture Regulate Fibrotic Gene Expression in Engineered Adipose Tissue.

Advanced Biosystems
Nikolas Di Caprio, Evangelia Bellas

Abstract

Adipose tissue (AT) has a dynamic extracellular matrix (ECM) surrounding adipocytes that allows for remodeling during metabolic fluctuations. During the progression of obesity, AT has increased ECM deposition, stiffening, and remodeling, resulting in a pro-fibrotic dysfunctional state. Here, the incorporation of ethylene glycol-bis-succinic acid N-hydroxysuccinimide ester (PEGDS) allows for control over 3D collagen hydrogel stiffness and architecture to investigate its influence on adipocyte metabolic and fibrotic function. Upon stiffening and altering ECM architecture, adipocytes did not alter their expression of key adipokines, leptin, and adiponectin. However, they do increase actin cytoskeletal fiber formation, pro-fibrotic gene expression, ECM deposition, and remodeling within a stiffer, 3D collagen hydrogel. For example, COL6A3 gene expression is upregulated approximately twofold, resulting in increased deposition of pericellular collagen VI alpha 3 surrounding adipocytes. Furthermore, inhibition of actin contractility results in a reversal of pro-fibrotic gene expression and ECM deposition, indicating that adipocytes are mediating mechanical cues through actin cytoskeletal networks. This study demonstrates that ECM stiff...Continue Reading

References

Apr 1, 1989·The Journal of Clinical Investigation·M D JensenJ M Miles
Aug 2, 2002·The New England Journal of Medicine·Satish KenchaiahRamachandran S Vasan
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Mar 3, 2007·The Journal of Clinical Investigation·Thomas A Wynn
Dec 31, 2008·Molecular and Cellular Biology·Tayeba KhanPhilipp E Scherer
Jun 24, 2009·Molecular and Cellular Biology·Nils HalbergPhilipp E Scherer
Sep 19, 2009·The Journal of Medical Investigation : JMI·Qinkai LiMakoto Funaki
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Magdalena PasaricaSteven R Smith
Jan 30, 2010·Nature·Daniel A Fletcher, R Dyche Mullins
Aug 25, 2010·The Journal of Cell Biology·Fei LiuDaniel J Tschumperlin
May 24, 2011·Acta Diabetologica·Soumaya KouidhiAmel Benammar Elgaaied
Apr 26, 2012·Nature Communications·Murat Guvendiren, Jason A Burdick
Feb 5, 2013·Tissue Engineering. Part C, Methods·Evangelia BellasDavid L Kaplan
Feb 26, 2013·Biochimica Et Biophysica Acta·Rebecca G Wells
Aug 21, 2013·Cell Metabolism·Kai SunPhilipp E Scherer
Mar 14, 2014·The Journal of Pathology·V PellegrinelliK Clément
Dec 1, 2015·Nature Materials·Ovijit ChaudhuriDavid J Mooney
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sungmin NamOvijit Chaudhuri
May 6, 2016·Frontiers in Endocrinology·Sung Sik ChoeJae Bum Kim
Jul 19, 2016·Biomaterials·Steven R CaliariJason A Burdick
Sep 28, 2017·Nature Reviews. Molecular Cell Biology·Tito PancieraStefano Piccolo
Oct 21, 2017·Metabolism: Clinical and Experimental·Emilie PastelKatarina Kos
Apr 14, 2018·Tissue Engineering. Part a·Robert D HumeChristine J Watson
Sep 27, 2019·Frontiers in Endocrinology·Samantha Costa, Michaela R Reagan
Jun 10, 2019·ACS Biomaterials Science & Engineering·Daniel L MateraBrendon M Baker

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Citations

Aug 23, 2020·International Journal of Molecular Sciences·Megan K DeBari, Rosalyn D Abbott
Oct 24, 2020·Cells·Hisham F BahmadMohamed Al-Sayegh
Jun 25, 2021·Nature Communications·Elina A PietiläKaisa Lehti
Jun 25, 2021·Advanced Science·Joanne X ShenVolker M Lauschke
Sep 3, 2021·Chemical Reviews·Franck J VernereyStephanie J Bryant
Nov 4, 2021·Scientific Reports·Golnaz Anvari, Evangelia Bellas

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