Collagen synthesis is not altered in women with stress urinary incontinence

Neurourology and Urodynamics
Yu ChenLeslie Kushner

Abstract

The objective of this study was to demonstrate that weakened pelvic floor support of the lower genitourinary tract in women with stress urinary incontinence (SUI) is due, in part, to decreased collagen synthesis and secretion and/or an altered ratio of collagen III/I synthesis by the fibroblasts of the endopelvic fascia and skin compared to that of women without evidence of pelvic floor weakening. Endopelvic fascia and skin biopsies were obtained from women with SUI (n = 14) and women without evidence of SUI or genital prolapse (n = 12). Fibroblast cultures established from the biopsies were incubated with 3H-proline in medium containing ascorbic acid for 3 hr. Conditioned medium was collected and cells were harvested. The radiolabeled collagens were precipitated and digested with collagenase. The collagen synthesized (as a percent of total protein) was determined. Collagen alpha1(III) was separated from collagen alpha1(I) and alpha2(I) by interrupted SDS-PAGE and the amount of (3)H-proline in each band was determined. Collagen synthesis, expressed as percent of total protein synthesis, was not significantly different between fibroblasts obtained from women with or without SUI. The mean of collagen III/I synthesized in fibrobla...Continue Reading

References

May 26, 1977·The New England Journal of Medicine·B SykesR Smith
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R P PenttinenV A McKusick
Oct 27, 1975·Biochemical and Biophysical Research Communications·A J BaileyS bazin
May 1, 1989·Human Genetics·A Superti-FurgaP H Byers
Sep 1, 1988·Archives of Biochemistry and Biophysics·F A BarileR S Bienkowski
Jan 1, 1987·Acta Obstetricia Et Gynecologica Scandinavica·U UlmstenA Malmström
Jul 1, 1972·Developmental Biology·R F Diegelmann, B Peterkofsky
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G S BarshP H Byers
Feb 1, 1982·Arthritis and Rheumatism·G R BotsteinE C Leroy
Sep 15, 1982·The Biochemical Journal·M AumailleyH Bricaud
Jan 1, 1994·Gynecologic and Obstetric Investigation·A BergmanM E Nimni
Jul 1, 1993·Obstetrical & Gynecological Survey·G Elia, A Bergman
May 1, 1993·European Journal of Obstetrics, Gynecology, and Reproductive Biology·T RechbergerJ Jakowicki
Oct 6, 1997·British Journal of Obstetrics and Gynaecology·D P KeaneA J Bailey
Dec 17, 1998·American Journal of Obstetrics and Gynecology·T RechbergerJ F Woessner
Oct 20, 1999·Current Opinion in Obstetrics & Gynecology·U Ulmsten, C Falconer
Jun 22, 2000·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·U KlingeB Klosterhalfen
Apr 12, 2001·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·U KlingeB Klosterhalfen
Jul 4, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·A LiapisG Creatsas
Jun 11, 2002·International Urogynecology Journal and Pelvic Floor Dysfunction·B H ChenM L Polan
Feb 28, 2003·Langenbeck's Archives of Surgery·R RoschV Schumpelick

❮ Previous
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Citations

Jun 2, 2006·International Urogynecology Journal and Pelvic Floor Dysfunction·Harold P Drutz, May Alarab
Apr 28, 2007·International Urogynecology Journal and Pelvic Floor Dysfunction·Andrew Feifer, Jacques Corcos
Aug 5, 2010·International Urogynecology Journal·Bernard JacquetinMichel Cosson
Apr 17, 2008·Hernia : the Journal of Hernias and Abdominal Wall Surgery·R C Read
Aug 27, 2005·Current Urology Reports·Matthew E KarlovskyGopal H Badlani
Aug 25, 2012·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Hemendra N Shah, Gopal H Badlani
Jan 10, 2008·International Urogynecology Journal and Pelvic Floor Dysfunction·Wassim BadiouRenaud de Tayrac
Mar 1, 2012·Actas urologicas españolas·M A AranhaG Ens
Sep 29, 2011·The Journal of Urology·Bertha Chen, Judy Yeh
Sep 3, 2011·BJU International·Lysanne CampeauKarl Erik Andersson
Sep 6, 2005·Urology·Matthew E KarlovskyGopal H Badlani
Apr 4, 2007·American Journal of Obstetrics and Gynecology·Wenjun ZongPamela A Moalli
May 5, 2009·The Journal of Pain : Official Journal of the American Pain Society·Michelle D SmithPaul W Hodges
Jun 11, 2016·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Sumerova NataliaPushkar Dmitri
Jul 23, 2013·The Journal of International Medical Research·Qiong HuangYong Zhou
Oct 15, 2014·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·Nadir I OsmanSheila MacNeil
Mar 29, 2012·Female Pelvic Medicine & Reconstructive Surgery·Dennis MillerDavid Robinson
Nov 3, 2006·Neurourology and Urodynamics·Kaytan V AmruteGopal H Badlani
Sep 22, 2007·Current Opinion in Obstetrics & Gynecology·Christian TwissLarissa V Rodríguez
Feb 16, 2006·Current Opinion in Urology·Kaytan V Amrute, Gopal H Badlani
May 10, 2007·Expert Review of Medical Devices·Uwe KlingeBernhard Schuessler
Feb 16, 2006·Current Opinion in Urology·Steven J Sowter, David A Tolley

❮ Previous
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