Collagen type I prevents glyoxal-induced apoptosis in osteoblastic cells cultured on titanium alloy

Cells, Tissues, Organs
S TippeltR H W Funk

Abstract

Advanced glycation end products (AGEs) irreversibly cross-link proteins with sugars and accumulate at a higher age and in diabetes, processes which can interfere with the integration of implants into the tissue. Glyoxal is a highly reactive glycating agent involved in the formation of AGEs and is known to induce apoptosis, as revealed by the upregulation of caspase-3 and fractin (caspase-3 being a key enzyme activated during the late stage of apoptosis and fractin being a caspase-cleaved actin fragment). In this study, we investigated the influence of collagen type I coating on the cytotoxic effect of glyoxal on rat calvarial osteoblastic cells and on human osteosarcoma cells (Saos-2) grown on titanium alloy, Ti6Al4V. Activation of caspase-3 and fractin was measured by counting immunohistochemically stained cells and by flow cytometry with propidium iodide (detection of the apoptosis indicating a sub-G1 peak). Our results showed an increased number of apoptotic osteoblasts after incubation with glyoxal on Ti6Al4V discs. However, the number of apoptotic cells on collagen-coated titanium was significantly smaller than on uncoated titanium after the same treatment. The present findings demonstrate that osteoblasts treated with gly...Continue Reading

References

Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Jul 1, 1995·Diabetes·J C KrakauerA M Parfitt
Jun 1, 1993·The Journal of Clinical Investigation·D R McCanceT J Lyons
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C KayalarD E Bredesen
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y KatayamaN Nagata
Dec 4, 1998·Stroke; a Journal of Cerebral Circulation·M R PuleraC G Wasterlain
Dec 30, 1998·Journal of Neural Transmission·C LoskeG Münch
Jun 25, 1999·Biochemical and Biophysical Research Communications·J K KrestowR S Kerbel
Jul 6, 2000·Journal of Biomedical Materials Research·U GeisslerK Wenzel
Aug 10, 2000·Neuropathology and Applied Neurobiology·J P RossiterG M Cole
Dec 29, 2000·Histochemistry and Cell Biology·C RoehleckeM Kasper
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I HunterS P Robins
Sep 29, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Z WagnerJ Nagy
Jan 5, 2002·Journal of Biomedical Materials Research·D BeckerK-W Wenzel
May 29, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Cyriel J A L MentinkBruce H R Wolffenbuttel
Dec 17, 2002·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Friedemann ReberRichard H W Funk
Mar 20, 2003·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Friedemann ReberRichard H W Funk

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Citations

Nov 19, 2009·Biomedical Engineering Online·Ying XueKamal Mustafa
Feb 25, 2011·Acta Biomaterialia·Limin Wang, Jan P Stegemann
Oct 16, 2012·Journal of Food Science·Chaim Frenkel, Thomas G Hartman
Mar 20, 2013·Biomatter·Susanne BierbaumDieter Scharnweber

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