Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas.

Experimental Eye Research
Christina S Kamma-LorgerKeith M Meek

Abstract

Corneal collagen ultrastructural changes occur during the healing process. The present study was designed to compare collagen ultrastructural changes after trephine wounding or flap creation. Bovine corneas were injured and maintained in organ culture for up to 4 weeks. Samples were removed from culture at 0, 1, 2, 3 and 4 weeks and snap frozen in liquid N(2). X-ray scattering was used to measure changes in collagen interfibrillar spacing, intermolecular spacing and fibrillar diameter. Some samples were fixed in 10% Neutral Buffered Formalin solution and wax embedded for immunohistochemistry to monitor myofibroblast differentiation in corneal flaps. Swelling effects (i.e. changes in interfibrillar spacing) were more severe in trephined corneas than in those with stromal flaps. Collagen fibrillar diameter remained normal in the periphery of injured corneas, but increased significantly in areas within and around the wound in trephined samples and in the epithelial incision site in corneal flaps. Intermolecular spacing was unchanged in all samples. In the flaps, alphaSMA expression was only detected in an area adjacent to the epithelial plug, and cell numbers gradually increased during the culture. We conclude that stromal swellin...Continue Reading

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Citations

May 8, 2013·PloS One·Mohammad AbahussinKeith M Meek
Jul 1, 2013·Asia-Pacific Journal of Ophthalmology·Lindsay A McGrath, Graham A Lee
Dec 25, 2010·Journal of Cataract and Refractive Surgery·Shengli MiChristina S Kamma-Lorger
Jan 4, 2012·Journal of Biomedical Materials Research. Part a·Lalitha MuthusubramaniamTejal A Desai
Mar 31, 2016·Journal of Cataract and Refractive Surgery·Siân R MorganKeith M Meek
Jul 22, 2015·Progress in Retinal and Eye Research·Alexander V Ljubimov, Mehrnoosh Saghizadeh
Apr 14, 2016·Tissue Engineering. Part C, Methods·Amy P LynchMark Ahearne
Mar 31, 2015·Experimental Eye Research·Andrew J QuantockJames V Jester
Jan 1, 2012·Journal of Functional Biomaterials·Samantha L WilsonYing Yang
Feb 2, 2019·Journal of Biomedical Materials Research. Part a·Monika PatelKazuaki Matsumura
Aug 17, 2020·Scientific Reports·Elena KoudounaPeter Lwigale
Jan 5, 2021·Anatomical Science International·Sandeepani K SubasingheGeorge J Dias

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