Collagen zymography as a sensitive and specific technique for the determination of subpicogram levels of interstitial collagenase

Analytical Biochemistry
B GoglyB Pellat

Abstract

We describe the use of casting native collagen type I in SDS-polyacrylamide gel (collagen zymography) for the determination of interstitial collagenase. As with gelatin, the incorporation of collagen in the gels reduced protein migration and the need for making corrections for an accurate Mr evaluation. This method proved to be very sensitive: 0.1 pg of APMA-activated procollagenase could be detected, and specific levels of active gelatinase or stromelysin lower than 5 ng were inactive under our experimental conditions. It was used to demonstrate the increased expression of collagenase following treatment of human gingival fibroblasts with interleukin-1 beta; the amounts of enzyme quantified by either collagen zymography or immunodot blot assay are comparable.

References

Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Matrisian

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Citations

Jun 24, 2010·The Journal of Biological Chemistry·Akiyoshi HoshinoKenji Yamamoto
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Juan Carlos JiménezMonique Capron
May 26, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Zhong-Dong ShiJohn M Tarbell
Jul 3, 1999·American Journal of Respiratory and Critical Care Medicine·G MautinoF Capony
Mar 19, 2013·PloS One·Nina E Lamash, Igor Yu Dolmatov
Apr 5, 2013·PloS One·Debashree DeDebasish Bhattacharyya
Apr 25, 2009·The American Journal of Pathology·Alan A ThomayJorge E Albina
Mar 18, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Xian-Chao ChengWen-Fang Xu
Dec 14, 2007·Connective Tissue Research·Adrien NaveauBruno Gogly
Feb 24, 2010·Journal of Pharmacological and Toxicological Methods·K KupaiP Ferdinandy
Dec 2, 2006·The Journal of Investigative Dermatology·Sylvie Igondjo-Tchen ChangotadeKarim Senni
Dec 13, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Stéphane JaissonPhilippe Gillery
Mar 23, 2005·Biochimie·Carine LombardJean Wallach
Apr 5, 2016·The Journal of Clinical Investigation·Jing FuSuzanne Lentzsch
Dec 3, 2014·European Journal of Pharmacology·Chien-Hsun HuangJoen-Rong Sheu
Mar 19, 2013·The American Journal of Pathology·Chun-Jun LiMing-Lin Liu
Sep 23, 2014·The American Journal of Chinese Medicine·Nam-Kyung ImGil-Saeng Jeong
Sep 2, 2003·Biochemical Pharmacology·Robert G BennettDean J Tuma
Jan 27, 2004·The American Journal of Pathology·Frédéric O MorvanJean-Louis Saffar
Oct 3, 2001·The Journal of Thoracic and Cardiovascular Surgery·M KanoT Kitagawa
Mar 19, 2013·Acta Cirúrgica Brasileira·Rodolfo Assis LisboaJosé Renan Cunha-Melo

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