Collagen/Heparin Bi-Affinity Multilayer Modified Collagen Scaffolds for Controlled bFGF Release to Improve Angiogenesis In Vivo

Macromolecular Bioscience
Wangping HaoJianwu Dai

Abstract

Basic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long-term controlled release of bFGF by utilizing a collagen/heparin bi-affinity multilayer delivery system (CHBMDS), which is fabricated by the alternate deposition of negatively charged heparin, positively charged collagen, and CBD-bFGF (a collagen-binding domain [CBD] was fused into the native bFGF) via specific or electrostatic interaction. The results show that CHBMDS not only support localized and prolonged release of CBD-bFGF(over 35 days) but also lead to enhanced angiogenesis (higher density and larger diameter (≈70 µm) of newly formed blood vessels in subcutaneous tissue of SD rat after 5 weeks). This system could act as a versatile approach for bFGF delivery and further improve therapeutic efficacy for injured tissues.

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Citations

Jun 10, 2020·Journal of Cellular and Molecular Medicine·Xiaoli HuJian Xiao
Apr 27, 2019·Frontiers in Bioengineering and Biotechnology·Francesco CopesDiego Mantovani
Apr 23, 2020·Journal of Biomedical Materials Research. Part a·Elizabeth A MaysHarini G Sundararaghavan
Feb 16, 2021·Frontiers in Cardiovascular Medicine·Bruna B J LealPatricia Pranke
May 27, 2021·Biotechnology and Bioengineering·Senthilkumar MuthusamyTong Cao
May 27, 2021·Biomedical Physics & Engineering Express·Zhenzhao GuoZhizhong Li

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