PMID: 3934504Jan 1, 1985

Collapse of cortex expansion during germination of Bacillus megaterium spores

Microbiology and Immunology
Y NakataniM Kondo

Abstract

When spores of Bacillus megaterium ATCC 12872 were incubated with CdCl2, they germinated without decomposition of the cortex. Moreover, the volume ratio of cortex to protoplast-plus-cortex, C/(P+C), of the CdCl2-germinated spores was reduced. Incubation of isolated cortex with the divalent compounds Cd2+, Ca2+, and Mg2+ reduced the gel volume to about 1/5 but incubation with a nonionic compounds, glucose, did not. The spores with reduced C/(P+C) were observed in the early period of glucose-induced germination. The time required for a 50% change in cortex morphology to occur was 2.5 min, which corresponds well with the time for 50% loss of heat resistance. This time was shorter than that necessary for release of peptidoglycan fragments and hydrolysis of cortex glycan chains. These data indicate that cortex hydrolysis is not related to the initiation of germination. 50% of the dipicolinic acid, calcium and magnesium were released at 3.4, 4.0, and 2.4 min, respectively. These results suggest that collapse of cortex expansion by the interaction of cortex with dipicolinic acid and cations released from the core, or exogenous ionic germinants is an important step in the initiation of germination.

References

Jun 1, 1977·The Journal of Applied Bacteriology·G W Gould
May 1, 1979·Journal of Bacteriology·D P Rossignol, J C Vary
May 1, 1979·Journal of Bacteriology·F M RacineJ C Vary
Jan 18, 1978·Biochimica Et Biophysica Acta·L K Shay, J C Vary
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Jan 1, 1985·Microbiology and Immunology·Y NakataniM Kondo
Jan 1, 1985·Microbiology and Immunology·Y NakataniM Kondo
Jan 1, 1982·Annual Review of Biochemistry·B M Conti-Tronconi, M A Raftery

Citations

Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L PophamP Setlow
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·K Johnstone
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·S J Foster
Jan 1, 1988·Microbiology and Immunology·M ImagawaM Kondo
Jan 1, 1987·Microbiology and Immunology·K WatabeM Torii
Jan 1, 1985·Microbiology and Immunology·Y NakataniM Kondo
Jan 1, 1985·Microbiology and Immunology·Y NakataniM Kondo

Related Concepts

Dipicolinic acid, monosodium salt
Bacillus megaterium
Cadmium
Calcium
Glucose, (beta-D)-Isomer
Magnesium
Electron Microscopy
Picolinic Acids
Protoplasts
Spores, Bacterial

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