Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile.

Molecular Neurobiology
Dominique LemaitreF A Court

Abstract

Peripheral nerve injuries result in motor and sensory dysfunction which can be recovered by compensatory or regenerative processes. In situations where axonal regeneration of injured neurons is hampered, compensation by collateral sprouting from uninjured neurons contributes to target reinnervation and functional recovery. Interestingly, this process of collateral sprouting from uninjured neurons has been associated with the activation of growth-associated programs triggered by Wallerian degeneration. Nevertheless, the molecular alterations at the transcriptomic level associated with these compensatory growth mechanisms remain to be fully elucidated. We generated a surgical model of partial sciatic nerve injury in mice to mechanistically study degeneration-induced collateral sprouting from spared fibers in the peripheral nervous system. Using next-generation sequencing and Ingenuity Pathway Analysis, we described the sprouting-associated transcriptome of uninjured sensory neurons and compare it with the activated by regenerating neurons. In vitro approaches were used to functionally assess sprouting gene candidates in the mechanisms of axonal growth. Using a novel animal model, we provide the first description of the sprouting ...Continue Reading

References

Feb 1, 1990·The Journal of Physiology·J Diaz, M Pécot-Dechavassine
Jun 1, 1994·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G LundborgJ M Kerns
Apr 4, 1998·The Journal of Comparative Neurology·K L LankfordJ D Kocsis
Feb 25, 2000·Experimental Neurology·R D MadisonG A Robinson
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·N WeidnerM H Tuszynski
Jul 12, 2002·Microscopy Research and Technique·Kazuho Hirata, Masaru Kawabuchi
Jan 6, 2004·Journal of Neurobiology·Eran PerlsonMike Fainzilber
Feb 18, 2004·Nature Neuroscience·Florence M BareyreMartin E Schwab
Aug 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Céline BouquetFatiha Nothias
Jul 24, 2007·Progress in Neurobiology·X NavarroAntoni Valero-Cabré
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rhona SeijffersClifford J Woolf
Apr 8, 2009·Experimental Neurology·Wilson Z Ray, Susan E Mackinnon
Jul 16, 2010·Science Signaling·Izhak MichaelevskiMike Fainzilber
May 2, 1903·British Medical Journal·C A BallanceP Stewart
Jan 21, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sebastian A BarrientosFelipe A Court
Feb 11, 2011·Developmental Neurobiology·Gianluca Gallo
Sep 20, 2011·Journal of the Neurological Sciences·Ping Yang, Zhong Yang
Jan 11, 2012·The Journal of Cell Biology·Jack T WangBen A Barres
Jan 11, 2013·Physiological Reviews·Jacqueline Cherfils, Mahel Zeghouf
Jan 1, 2013·Brain Sciences·Asa Fex Svennigsen, Lars B Dahlin
Sep 2, 2014·BioMed Research International·Eric P KnottDamien D Pearse
Oct 27, 2015·Nature Neuroscience·Songlin LiS Thomas Carmichael
Feb 24, 2016·Neuron·Vijayendran ChandranDaniel H Geschwind
Oct 27, 2016·Orthopedics·Georgios N PanagopoulosAndreas F Mavrogenis
Mar 2, 2017·International Journal of Molecular Sciences·Xin-Peng Dun, David B Parkinson
Mar 16, 2017·Cell Reports·Kathren L FinkWilliam B J Cafferty
Jul 12, 2017·Journal of Korean Neurosurgical Society·Soner CiviSukru Aykol
Nov 10, 2017·Cell Death & Disease·Alejandra CatenaccioFelipe A Court

❮ Previous
Next ❯

Citations

Jan 30, 2021·Neural Regeneration Research·Dominique Lemaitre, Felipe A Court

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
electron microscopy
light microscopy
mechanical dissociation
PCR
RNA-seq
nucleotide exchange

Software Mentioned

Origene
Ingenuity Pathway Analysis (
Cufflinks
AxReg
ColSpr
Fast QC ( Control Tool

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.