Colletotrichum orbiculare Regulates Cell Cycle G1/S Progression via a Two-Component GAP and a GTPase to Establish Plant Infection

The Plant Cell
Fumi Fukada, Yasuyuki Kubo

Abstract

Morphogenesis in filamentous fungi depends on appropriate cell cycle progression. Here, we report that cells of the cucumber anthracnose fungus Colletotrichum orbiculare regulate G1/S progression via a two-component GAP, consisting of Budding-uninhibited-by-benomyl-2 (Bub2) and Byr-four-alike-1 (Bfa1) as well as its GTPase Termination-of-M-phase-1 (Tem1) to establish successful infection. In a random insertional mutagenesis screen of infection-related morphogenesis, we isolated a homolog of Saccharomyces cerevisiae, BUB2, which encodes a two-component Rab GAP protein that forms a GAP complex with Bfa1p and negatively regulates mitotic exit. Interestingly, disruption of either Co BUB2 or Co BFA1 resulted in earlier onset of nuclear division and decreased the time of phase progression from G1 to S during appressorium development. S. cerevisiae GTPase Tem1p is the downstream target of the Bub2p/Bfa1p GAP complex. Introducing the dominant-negative form of Co Tem1 into Co bub2Δ or Co bfa1Δ complemented the defect in G1/S progression, indicating that Co Bub2/Co Bfa1 regulates G1/S progression via Co Tem1. Based on a pathogenicity assay, we found that Co bub2Δ and Co bfa1Δ reduced pathogenesis by attenuating infection-related morphoge...Continue Reading

Citations

Dec 26, 2015·Fungal Genetics and Biology : FG & B·Magdalena Martin-UrdirozNicholas J Talbot
Apr 2, 2016·Seminars in Cell & Developmental Biology·José Pérez-MartínMaría Tenorio-Gómez
Dec 4, 2019·ELife·Míriam Osés-Ruiz, Nicholas J Talbot
Nov 10, 2020·Science China. Life Sciences·Pengjie HuLinqi Wang
Nov 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Antonio de la TorreJosé Pérez-Martín
Mar 18, 2021·MBio·Sayumi FukudaNorio Takeshita

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