Colocalisation of insulin and IGF-1 receptors in cultured rat sensory and sympathetic ganglion cells

Journal of Anatomy
S N KaragiannisP K Thomas

Abstract

Peripheral sensory and autonomic neurons are known to possess insulin receptors. These have been considered to be of the peripheral type, i.e. similar to those of hepatic and fat cells rather than of the brain type which show dual specificity for both insulin and insulin-like growth factor (IGF-1). We have examined the localisation of insulin and IGF-1 receptors in cultured sensory and sympathetic ganglion cells using confocal microscopy and indirect labelling with FITC (fluorescein isothiocyanate) and TRITC (tetramethyl rhodamine isothiocyanate) respectively. We have shown that in cultured U266B1 multiple myeloma cells these receptors display separate localisation, whereas they are colocalised in IM-9 lymphocytes which are known to possess hybrid receptors. We have confirmed the sequestration of insulin and IGF-1 receptors in the cytoplasm of sensory and sympathetic neurons, consistent with a brain-type receptor. The colocalisation of insulin and IGF-1 receptors in sensory and sympathetic ganglion cells is consistent with the view that they are hybrid receptors, similar to those present in the CNS. The function of these receptors, as suggested for the CNS, may be related to trophic support for neurons.

References

Jan 1, 1977·The Journal of Clinical Investigation·M B Davidson, S A Kaplan
Dec 1, 1979·Endocrinology·S T Pacold, W G Blackard
Sep 1, 1976·Journal of the Neurological Sciences·J M JacobsJ B Cavanagh
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Jan 29, 1986·Biochemical and Biophysical Research Communications·W L Lowe, D LeRoith
Apr 1, 1988·Neurochemical Research·M K RaizadaD LeRoith
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M Smith, L Jarett
Sep 1, 1986·The Journal of Clinical Endocrinology and Metabolism·K Tan, R C Baxter
May 19, 1972·Science·G Vann Bennett, P Cuatrecasas
Sep 14, 1984·Science·D G Puro, E Agardh
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E Recio-PintoD N Ishii
Jan 1, 1984·Journal of Neurochemistry·M KappyM Raizada
Nov 1, 1984·Journal of Neurochemistry·S A HendricksJ Roth
Dec 1, 1983·Brain : a Journal of Neurology·G SaidJ Selva
Oct 31, 1980·Biochemical and Biophysical Research Communications·C C YipC W Yeung
Oct 1, 1994·Diabetic Medicine : a Journal of the British Diabetic Association·P K Thomas
Feb 1, 1994·Journal of the Neurological Sciences·N J PatelP K Thomas
Aug 27, 1993·Annals of the New York Academy of Sciences·K A Heidenreich
Jan 1, 1993·Annual Review of Physiology·W S Cohick, D R Clemmons

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Citations

Jan 4, 2008·Neuromolecular Medicine·Mark P Mattson, Ruiqian Wan
Sep 26, 2002·Cell Research·Deepak ChhabraCristobal G dos Remedios
Aug 23, 2008·Endocrinology·Kelli A SullivanEva L Feldman
Feb 7, 2012·Journal of Neurochemistry·Bhagat SinghDouglas W Zochodne
Dec 3, 2014·Neuroscience Letters·Douglas W Zochodne
Feb 7, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·B R LackeyD M Henricks
Apr 12, 2007·International Anesthesiology Clinics·Maxim DobretsovJoseph R Stimers
Nov 12, 2003·The Journal of Pharmacology and Experimental Therapeutics·Fiona R CoulsonAllison D Fryer
Nov 7, 1999·The American Journal of Pathology·R E SchmidtM S Miller

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