Colocalization of antisense RNAs and ribozymes with their target mRNAs

Genome Génome / Conseil National De Recherches Canada
G M Arndt, G H Rank

Abstract

The use of complementary RNA sequences such as antisense RNAs and ribozymes to regulate the expression of specific genes in eukaryotic cells has been well-documented, particularly with their application to both human gene therapy and plant biotechnology. Despite the simplicity of this approach, this technique usually results in only partial suppression of gene expression and, in some instances, even fails to regulate the gene of interest. The variation observed with antisense RNA and ribozyme-mediated regulation is further complicated by the many factors with the potential to impact on the effectiveness of these RNAs. Recent advances in the understanding of the global architecture of the nucleus, chromatin structure, and RNA metabolism provide useful and necessary information for designing novel approaches to improving antisense RNA and ribozyme regulation. These studies predict that the position of genes within the nucleus is not random and that transcripts produced from these genes follow specific tracks in migrating to the cell cytoplasm. These observations have the potential to impact significantly on the ways in which RNA-mediated forms of gene regulation are applied. The purpose of this review is to discuss the concept of...Continue Reading

References

Apr 17, 1992·Cell·C M DeomR N Beachy
Apr 15, 1992·Gene·R PerrimanW L Gerlach
Jan 11, 1991·Nucleic Acids Research·M GraessmannA Graessmann
Sep 1, 1985·The Journal of Cell Biology·R Harland, H Weintraub
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W M Wormington
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A BevilacquaV Hieber
Apr 1, 1988·Current Genetics·W Xiao, G H Rank
Aug 15, 1989·Biochemical and Biophysical Research Communications·J C Chuat, F Galibert
Jan 1, 1986·Gene·L G KralT B Friedman
Feb 1, 1987·Molecular and Cellular Biology·K Nishikura, J M Murray
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D A Melton
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Aug 21, 1995·Molecular & General Genetics : MGG·G M ArndtJ G Izant
Mar 21, 1995·Gene·G FerbeyreR Cedergren
Dec 1, 1994·Current Opinion in Biotechnology·K C Carter
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M H KumagaiL K Grill
Mar 1, 1994·Current Genetics·G M ArndtG H Rank
Jan 1, 1994·Antisense Research and Development·D Atkins, W L Gerlach
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·E WaigmannP Zambryski
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z LiuG G Carmichael
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Apr 1, 1997·Current Opinion in Genetics & Development·W F MarshallJ W Sedat
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R E SheehyW R Hiatt

❮ Previous
Next ❯

Citations

Nov 20, 1998·Current Opinion in Biotechnology·P J WelchF Wong-Staal
Oct 13, 2006·Current Opinion in Infectious Diseases·A J SteynV Mehra
Apr 6, 2000·Current Opinion in Oncology·S J Antonia, E Sotomayor
Jan 22, 2004·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Isabelle Gautherot, Regís Sodoyer
Mar 22, 2002·Cancer Control : Journal of the Moffitt Cancer Center·Maria G SotomayorDrew M Pardoll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.