PMID: 10805922May 11, 2000

Colocalization of multiple GABA(A) receptor subtypes with gephyrin at postsynaptic sites

The Journal of Comparative Neurology
Marco Sassoè-PognettoJ M Fritschy

Abstract

Clustering of gamma aminobutyric acid (GABA)(A) receptors to postsynaptic sites requires the presence of both the gamma2 subunit and gephyrin. Here, we analyzed by double-immunofluorescence staining the colocalization of gephyrin and major GABA(A)-receptor subtypes distinguished by the subunits alpha1, alpha2, alpha3, or gamma2 in adult rat brain. By using confocal laser scanning microscopy, GABA(A)-receptor subunit staining revealed brightly stained clusters that were colocalized with gephyrin-positive clusters of similar size and distribution in several brain regions, including cerebellum, hippocampus, thalamus, and olfactory bulb. In addition, a diffuse staining was observed for GABA(A)-receptor subunits in the neuropil, presumably representing extrasynaptic receptors. Overall, only few gephyrin-positive clusters were not colocalized with GABA(A)-receptor subunit clusters. Electron microscopic analysis in cerebellar cortex confirmed the selective postsynaptic localization of gephyrin. High-resolution images (voxel size, 50 x 50 x 150 nm) were restored with an iterative image deconvolution procedure based on a measured point-spread function to analyze the colocalization between GABA(A)-receptor subunits and gephyrin in indivi...Continue Reading

References

Mar 13, 2013·Brain Structure & Function·Pablo HennyJ Paul Bolam
Jul 2, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·C A KellerB Lüscher
Aug 15, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark D EyreZoltan Nusser
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Verena TretterStephen J Moss
Mar 2, 2011·Nature Neuroscience·Marian W GlynnA Kimberley McAllister
Sep 2, 2011·The Journal of Biological Chemistry·Verena TretterKirsten Harvey
Oct 19, 2011·The Journal of Biological Chemistry·Hans-Michael MaricHermann Schindelin
Jul 1, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Cyrille VaillendElise Peltekian
Aug 18, 2000·The European Journal of Neuroscience·Marco Sassoè-Pognetto, J M Fritschy
Jan 28, 2005·The European Journal of Neuroscience·Carolien van RijnsoeverJean-Marc Fritschy
Feb 18, 2005·The Journal of Comparative Neurology·Patrizia PanzanelliMarco Sassoè-Pognetto
Jan 17, 2002·The Journal of Comparative Neurology·Ina BrünigJean-Marc Fritschy
Mar 23, 2010·Neurobiology of Disease·Zakaria MtchedlishviliKevin M Kelly
Aug 29, 2013·The Journal of Comparative Neurology·Alexander E LinsalataThomas S Reese
Dec 22, 2004·The European Journal of Neuroscience·Emilie MullerPascal Legendre
May 7, 2004·The European Journal of Neuroscience·B HutcheonM O Poulter
Apr 12, 2007·The European Journal of Neuroscience·Marie-Madeleine GabellecPierre-Marie Lledo
Feb 1, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zhihong LiJinlian Li
Dec 25, 2009·The Journal of Comparative Neurology·Khushdev K ThindPaul S Buckmaster
Dec 23, 2014·Nature Communications·Hans Michael MaricHermann Schindelin
Jul 15, 2015·Experimental Neurology·Daniel J RaibleAmy R Brooks-Kayal
Sep 22, 2009·Psychoneuroendocrinology·Nadezhda N ZheleznovaDavid S Weiss
Oct 5, 2001·Nature Cell Biology·B Lüscher, C A Keller
Apr 18, 2015·Experimental & Molecular Medicine·Gayoung Choii, Jaewon Ko
Aug 12, 2004·The Journal of Comparative Neurology·Jean-Marc FritschyDietmar Benke
Mar 17, 2015·The Journal of Comparative Neurology·Francine DeprezJean-Marc Fritschy
Apr 24, 2012·The Journal of Comparative Neurology·Jolly PaulJean-Marc Fritschy
Jul 26, 2012·Journal of Neurotrauma·Daniel J RaibleAmy R Brooks-Kayal

Citations

Oct 15, 1988·The Journal of Comparative Neurology·R J WentholdR A Altschuler
Aug 1, 1985·The Journal of Cell Biology·Antoine TrillerH Korn
Jan 1, 1988·Anatomy and Embryology·R L Jakab, J Hámori
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F PfeifferH Betz
Aug 28, 1995·Brain Research. Developmental Brain Research·B Gao, J M Fritschy
Jun 19, 1995·The Journal of Comparative Neurology·Marco Sassoè-PognettoH Wässle
Jan 1, 1995·The European Journal of Neuroscience·A J ToddM Neilson
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Dieudonné
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Zoltan NusserP Somogyi
Jan 1, 1996·Molecular and Cellular Neurosciences·J KirschH Betz
Mar 3, 1997·The Journal of Comparative Neurology·F J AlvarezR E Fyffe
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Gregg E HomanicsR W Olsen
Feb 17, 1998·The European Journal of Neuroscience·O P Ottersen, A S Landsend
Mar 5, 1998·Neuroscience Letters·M D KrasowskiGregg E Homanics
May 29, 1998·The Journal of Comparative Neurology·M GiustettoMarco Sassoè-Pognetto
Jul 22, 1999·Annals of the New York Academy of Sciences·P J WhitingKeith Wafford
Dec 14, 1999·The European Journal of Neuroscience·I KnueselJ M Fritschy

Related Concepts

Establishment and Maintenance of Localization
Immunofluorescence Assay
GPHN gene
Neuropil
Structure of Olfactory Bulb
Brain
Gephyrin
Immunocytochemistry
GABA-A Receptor
Optical Image Reconstruction

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.