PMID: 9542531Jul 1, 1997Paper

Colocalization of P2Y2 and P2Y6 receptor genes at human chromosome 11q13.3-14.1

Somatic Cell and Molecular Genetics
L V PidlaoanS P Kunapuli

Abstract

Extracellular nucleotides mediate a number of physiological responses through either ligand gated P2X or G protein-coupled P2Y receptors. To date, six P2Y receptor subtypes, P2Y1-P2Y6, have been cloned. We mapped the human P2Y6 receptor gene to chromosome 11q13.3-13.5. Oligonucleotide primers complementary to a part of the human P2Y6 receptor cDNA were used to amplify a region from genomic DNA from a panel of mouse/human somatic cell hybrid cell lines, each containing a single human chromosome. A PCR product of the expected size (714 bp) resulted from a single hybrid cell line containing human chromosome 11. The gene was further localized to a region of chromosome 11 using a subchromosomal hybrid panel containing different segments of chromosome 11. Based on the specific PCR product obtained and its Southern hybridization to the P2Y6 receptor cDNA, the human P2Y6 receptor gene was localized to chromosome 11q13.3-13.5. Previously, we have localized the P2Y2 receptor gene to human chromosome 11q13.5-14.1. This is the first report of the clustering of the P2 receptor genes. The clustering of these two P2Y receptor subtypes suggests a relatively recent expansion of the gene family by gene duplication.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1989·Somatic Cell and Molecular Genetics·T GlaserC Jones
Nov 3, 1995·The Journal of Biological Chemistry·K ChangY Takuwa
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K D LustigD Julius
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E ParrJ T Turner
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C E ParrJ T Turner
Dec 29, 1995·The Journal of Biological Chemistry·T NguyenB F O'Dowd
Dec 29, 1995·The Journal of Biological Chemistry·D CommuniJ M Boeynaems
Jan 26, 1996·Biochemical and Biophysical Research Communications·K AyyanathanS P Kunapuli
Feb 6, 1996·Biochemical and Biophysical Research Communications·T E WebbE A Barnard
Jan 1, 1996·Somatic Cell and Molecular Genetics·V R DasariS P Kunapuli
May 15, 1996·Biochemical and Biophysical Research Communications·D CommuniJ M Boeynaems
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·C MollereauM Parmentier
May 1, 1996·Journal of Receptor and Signal Transduction Research·G K AkbarS P Kunapuli
Sep 1, 1996·Somatic Cell and Molecular Genetics·K AyyanathanS P Kunapuli

❮ Previous
Next ❯

Citations

May 19, 2009·Neuroscience Bulletin·Gui-Dong LiuSheng-Di Chen
Apr 16, 1998·British Journal of Pharmacology·J JinS P Kunapuli
Jul 28, 1999·Clinical and Experimental Hypertension : CHE·P Hamet
Feb 24, 2006·Pharmacogenetics and Genomics·Rainer BüscherPaul A Insel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.