Colocalization of somatostatin receptors and epidermal growth factor receptors in breast cancer cells

Cancer Cell International
Heather L Watt, Ujendra Kumar

Abstract

Somatostatin receptor (SSTR) expression is positively correlated with tumor size and inversely correlated with epidermal growth factor receptor (ErbB) levels and tumor differentiation. In the present study, we compared SSTR1-5 and ErbB1-4 mRNA and protein expression in two breast cancer cell lines: MCF-7 (ER+) and MDA-MB-231 (ERalpha-). All five SSTRs and four ErbBs were variably expressed as both cell surface and cytoplasmic proteins. In both cell lines, SSTR4 and SSTR1 were highly expressed, followed by SSTR2 and SSTR5 with SSTR3 being the least expressed subtype, at the protein level. ErbBs were variably expressed with ErbB1 as the predominant subtype in both cell lines. ErbB1 is followed by ErbB3, ErbB2 and ErbB4 in MCF-7 at both the protein and mRNA levels. In MDA-MB-231 cells, ErbB1 is followed by ErbB2, ErbB4 and ErbB3. Our results indicate significant correlations at the level of mRNA and protein expression in a cell and receptor-specific manner. Using indirect immunofluorescence, we found that, in MCF-7 cells, SSTR5 was the most prominent subtype coexpressed with ErbBs followed by SSTR3, SSTR4, SSTR1 and SSTR2, respectively. In MDA-MB-231 cells, SSTR1 colocalized strongly with ErbBs followed by SSTR5, SSTR4, SSTR3 and ...Continue Reading

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Citations

Jul 25, 2008·Reviews in Endocrine & Metabolic Disorders·Alexandru Saveanu, Philippe Jaquet
Jul 4, 2009·Acta Pharmacologica Sinica·Yong HeGuan-Xin Shen
Apr 12, 2008·Cancer Gene Therapy·G Sithanandam, L M Anderson
Oct 1, 2019·Anti-cancer Agents in Medicinal Chemistry·Aysegul HanikogluCarla Ferreri

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Methods Mentioned

BETA
PCR
ubiquitination
electrophoresis

Software Mentioned

Adobe Photoshop

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