Colocalization of the (Pro)renin Receptor/Atp6ap2 with H+-ATPases in Mouse Kidney but Prorenin Does Not Acutely Regulate Intercalated Cell H+-ATPase Activity

PloS One
Arezoo DaryadelCarsten A Wagner

Abstract

The (Pro)renin receptor (P)RR/Atp6ap2 is a cell surface protein capable of binding and non-proteolytically activate prorenin. Additionally, (P)RR is associated with H(+)-ATPases and alternative functions in H(+)-ATPase regulation as well as in Wnt signalling have been reported. Kidneys express very high levels of H(+)-ATPases which are involved in multiple functions such as endocytosis, membrane protein recycling as well as urinary acidification, bicarbonate reabsorption, and salt absorption. Here, we wanted to localize the (P)RR/Atp6ap2 along the murine nephron, exmaine whether the (P)RR/Atp6ap2 is coregulated with other H(+)-ATPase subunits, and whether acute stimulation of the (P)RR/Atp6ap2 with prorenin regulates H(+)-ATPase activity in intercalated cells in freshly isolated collecting ducts. We localized (P)PR/Atp6ap2 along the murine nephron by qPCR and immunohistochemistry. (P)RR/Atp6ap2 mRNA was detected in all nephron segments with highest levels in the collecting system coinciding with H(+)-ATPases. Further experiments demonstrated expression at the brush border membrane of proximal tubules and in all types of intercalated cells colocalizing with H(+)-ATPases. In mice treated with NH4Cl, NaHCO3, KHCO3, NaCl, or the mi...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R D NelsonS Gluck
Nov 4, 1985·Nature·G J SchwartzQ Al-Awqati
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L AlperD Brown
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S GluckQ Al-Awqati
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Sep 1, 1994·Pflügers Archiv : European journal of physiology·M GekleS Silbernagl
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A WagnerJ P Geibel
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·I E RoyauxE D Green
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Tsuyoshi Nishi, Michael Forgac
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Young-Hee KimSøren Nielsen
May 16, 2003·Pflügers Archiv : European journal of physiology·Carsten A WagnerJohn P Geibel
Sep 27, 2003·The Journal of Biological Chemistry·Nuria Pastor-SolerSylvie Breton
Nov 26, 2003·Journal of the American Society of Nephrology : JASN·Paul A StehbergerCarsten A Wagner
Feb 6, 2004·Pflügers Archiv : European journal of physiology·Brenda Bonnici, Carsten A Wagner
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christian WinterCarsten A Wagner
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel
Apr 6, 2007·Journal of the American Society of Nephrology : JASN·Paul A StehbergerCarsten A Wagner
Jun 28, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nicole SchulzCarsten A Wagner
Dec 7, 2007·Physiological Genomics·Marta NowikCarsten A Wagner
Mar 7, 2008·American Journal of Physiology. Renal Physiology·Carsten A WagnerJohannes Loffing
Oct 31, 2008·American Journal of Physiology. Cell Physiology·Patricia HafnerCarsten A Wagner
Nov 21, 2008·Nature·Sophie BiverAnna Maria Marini
Nov 18, 2009·Journal of the American Society of Nephrology : JASN·Genevieve Nguyen, Dominik N Muller
Jan 8, 2010·American Journal of Physiology. Renal Physiology·Teodor G PăunescuDennis Brown
Feb 12, 2010·American Journal of Physiology. Renal Physiology·Fan GongNúria M Pastor-Soler
May 28, 2010·Kidney International·Gabin SihnMichael Bader
Jun 8, 2010·The Journal of Biological Chemistry·Rodrigo AlzamoraNúria M Pastor-Soler
Jun 29, 2010·Current Biology : CB·Tobias HermleMatias Simons
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Marta NowikCarsten A Wagner
Oct 29, 2011·Journal of the American Society of Nephrology : JASN·Fabian RiedigerDominik N Muller
Nov 5, 2011·Journal of the American Society of Nephrology : JASN·Yoichi OshimaHiroshi Itoh

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Citations

Jul 22, 2016·American Journal of Physiology. Renal Physiology·Chuanming XuTianxin Yang
Mar 9, 2017·Journal of the American Society of Nephrology : JASN·Zhen LiYouhua Liu
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Katrina J BingerAndreas L Birkenfeld
Sep 26, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yanting Chen, Chuanming Xu
Jul 3, 2018·Pflügers Archiv : European journal of physiology·Arezoo DaryadelCarsten A Wagner
Sep 30, 2016·American Journal of Physiology. Renal Physiology·Nirupama Ramkumar, Donald E Kohan
Jul 7, 2021·Pflügers Archiv : European journal of physiology·Marta FigueiredoCarsten A Wagner

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Methods Mentioned

BETA
PCR
Assay
protein Assay
dissection
light microscopy

Software Mentioned

Advanced Image Data Analyzer

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