Colocalization prostacyclin (PGI2) synthase--caveolin-1 in endothelial cells and new roles for PGI2 in angiogenesis

Experimental Cell Research
Enzo SpisniVittorio Tomasi

Abstract

In vascular cells, prostacyclin (PGI2) synthase (PGI2s) has been localized in the endoplasmic reticulum of endothelial cells and in the nuclear and plasma membrane of smooth muscle cells. In human umbilical vein endothelial (HUVE) cells, we detected the enzyme in abundant cytoplasmic vesicles apparently originating from the plasma membrane and similar to those stained by gold-albumin, which interacts with a caveolar receptor. This prompted us to try a direct confocal microscopy approach aimed at colocalizing gold-albumin, caveolin-1, and PGI2 synthase. Moreover, the staining of HUVE cells with an anti-BiP7Grp78 antibody (a marker of endoplasmic reticulum) shows a perinuclear localization, sharply separated from PGI2 synthase localization. The results indicate that more than 80% of the enzyme resides in cellular sites costaining with caveolin-1 antibody and gold-albumin. This evidence was confirmed by the demonstration that PGI2 synthase and caveolin-1 coimmunoprecipitate in HUVE cell lysates and that they are associated to detergent-insoluble membrane domains in the same low-density fractions of a sucrose gradient. In addition, depletion of cellular cholesterol by mevalonate and methyl-beta-cyclodextrin leads to the shift of PG...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B B WekslerE A Jaffe
Oct 1, 1983·Physiological Reviews·N Simionescu
Oct 26, 1995·Biochimica Et Biophysica Acta·M NegishiA Ichikawa
Jan 1, 1995·American Journal of Respiratory Cell and Molecular Biology·J J ZuluetaP M Hassoun
Apr 26, 1995·Biochemical and Biophysical Research Communications·B BellettiV Tomasi
Aug 30, 1994·Biochemical and Biophysical Research Communications·B PereiraL H Wang
May 16, 1994·Biochemical and Biophysical Research Communications·A MiyataT Tanabe
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C TiruppathiA B Malik
Aug 1, 1996·Current Opinion in Cell Biology·R G Parton
Nov 1, 1996·The Journal of Biological Chemistry·G García-CardeñaW C Sessa
Nov 15, 1996·The Journal of Biological Chemistry·E J SmartR G Anderson
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B M FormanR M Evans
Jun 20, 1997·The Journal of Biological Chemistry·J B MichelT Michel
Mar 21, 1998·The Journal of Cell Biology·S EsserW Risau
Apr 29, 1998·The Journal of Cell Biology·P Keller, K Simons
May 23, 1998·The Journal of Biological Chemistry·A G SpencerW L Smith
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·B F McAdamG A FitzGerald
Apr 17, 1999·The Journal of Biological Chemistry·T G BrockM Peters-Golden
Oct 19, 1999·Molecular and Cellular Biology·E J SmartM P Lisanti

❮ Previous
Next ❯

Citations

Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Hong Pyo KimAugustine M K Choi
Jun 11, 2011·Pulmonary Medicine·Rajamma Mathew
Jun 26, 2012·Trends in Pharmacological Sciences·Zvonimir S KatusicTongrong He
May 5, 2009·Journal of Molecular and Cellular Cardiology·Travis W HeinLih Kuo
Feb 11, 2012·The American Journal of Pathology·Christudas MoraisMarie-Odile Parat
Jul 21, 2015·Microvascular Research·Khuyen Gia HoangNenad Petrovic
May 6, 2015·Peptides·Thomas P DavisRichard D Egleton
Jul 6, 2004·European Journal of Biochemistry·Inger SchousboeBo van Deurs
Sep 23, 2003·Journal of Cellular Physiology·Enzo SpisniVittorio Tomasi
Nov 18, 2003·Traffic·Masashi Isshiki, Richard G W Anderson
May 10, 2007·Journal of Cellular and Molecular Medicine·Cristiana GriffoniVittorio Tomasi
Jun 12, 2013·Clinical and Experimental Pharmacology & Physiology·Jayanth RamadossRonald R Magness
Nov 3, 2005·Biochemical and Biophysical Research Communications·Enzo SpisniSilvio C E Tosatto
Aug 24, 2005·Biochemical and Biophysical Research Communications·Kenneth K Wu, Jun-Yang Liou
May 10, 2007·Journal of Biomedicine & Biotechnology·Mattia ToniVittorio Tomasi
Mar 9, 2005·Biochemical and Biophysical Research Communications·Chunying LiRichard C Venema
Feb 4, 2010·Biochimica Et Biophysica Acta·Mary-Clare CathcartGraham P Pidgeon
Apr 20, 2006·British Journal of Cancer·A StrillacciV Tomasi
Apr 10, 2013·International Journal of Molecular Sciences·Andreas DaiberVolker Ullrich
Apr 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Philippe G FrankMichael P Lisanti
Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angels NavarroMarie-Odile Parat
Aug 2, 2003·Nature Reviews. Cancer·Lucy A Carver, Jan E Schnitzer
Apr 3, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Markus BachschmidVolker Ullrich
May 10, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefan SchildknechtVolker Ullrich
May 30, 2002·The Journal of Biological Chemistry·Hiromichi MatsumotoSudhansu K Dey
Oct 14, 2018·Cell Death & Disease·Mengxin LuXinghuan Wang
Feb 18, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·L AlibardiC Taddei
May 19, 2006·Reproductive Medicine and Biology·Hiromichi Matsumoto, Eimei Sato
Jan 10, 2002·Cellular Signalling·Maria Lina MassiminoVittorio Tomasi
Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Rachel J A HelliwellMurray D Mitchell
Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Rachel J A HelliwellMurray D Mitchell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

© 2021 Meta ULC. All rights reserved