Colon dysregulation in methamphetamine self-administering HIV-1 transgenic rats

PloS One
Amanda L PersonsT Celeste Napier

Abstract

The integrity and function of the gut is impaired in HIV-infected individuals, and gut pathogenesis may play a role in several HIV-associated disorders. Methamphetamine is a popular illicit drug abused by HIV-infected individuals. However, the effect of methamphetamine on the gut and its potential to exacerbate HIV-associated gut pathology is not known. To shed light on this scenario, we evaluated colon barrier pathology in a rat model of the human comorbid condition. Intestinal barrier integrity and permeability were assessed in drug-naïve Fischer 344 HIV-1 transgenic (Tg) and non-Tg rats, and in Tg and non-Tg rats instrumented with jugular cannulae trained to self-administer methamphetamine or serving as saline-yoked controls. Intestinal permeability was determined by measuring the urine content of orally gavaged sugars. Intestinal barrier integrity was evaluated by immunoblotting or immunofluorescence of colon claudin-1 and zonula occludens-1 (ZO-1), two major tight junction proteins that regulate gut epithelial paracellular permeability. Both non-Tg and Tg rats self-administered moderate amounts of methamphetamine. These amounts were sufficient to increase colon permeability, reduce protein level of claudin-1, and reduce cl...Continue Reading

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Citations

Apr 9, 2020·AIDS and Behavior·Adam W CarricoSabina Hirshfield
Jun 23, 2018·Brain Structure & Function·Michael Ohene-NyakoT Celeste Napier
Jul 30, 2020·The European Journal of Neuroscience·Michael Ohene-NyakoT Celeste Napier
Apr 11, 2021·Neuroscience Letters·Thomas J Cirino, Jay P McLaughlin
Aug 26, 2021·Human Psychopharmacology·Yayan LuoNi Fan

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Methods Mentioned

BETA
transgenic
sedation

Software Mentioned

ImageJ
GraphPad Prism
GB
GraphPad
Stat

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