Colonization and Movement of Xanthomonas fragariae in Strawberry Tissues

Phytopathology
Hehe WangW W Turechek

Abstract

Xanthomonas fragariae causes angular leaf spot of strawberry, an important disease in strawberry growing regions worldwide. To better understand how X. fragariae multiplies and moves in strawberry plants, a green fluorescent protein (GFP)-labeled strain was constructed and used to monitor the pathogen's presence in leaf, petiole, and crown tissue with fluorescence microscopy following natural and wound inoculation in three strawberry cultivars. Taqman PCR was used to quantify bacterial densities in these same tissues regardless of the presence of GFP signal. Results showed X. fragariae colonized leaf mesophyll, the top 1 cm portion of the petiole adjacent to the leaf blade, and was occasionally found colonizing xylem vessels down to the middle of the petioles. The colonization of vascular bundles and the limited systemic movement that was observed appeared to be a passive process, of which the frequency increased with wounding and direct infiltration of bacteria into leaf veins. X. fragariae was able to directly enter petioles and colonize the space under the epidermis. Systemic movement of the bacteria into crown and other uninoculated tissues was not detected visually by GFP. However, X. fragariae was occasionally detected in...Continue Reading

References

May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·C C Li
Nov 15, 1996·FEMS Microbiology Letters·A G MatthysseA E Goodman
Apr 22, 2008·Annual Review of Phytopathology·Maeli MelottoSheng Yang He
Apr 12, 2011·Nature Reviews. Microbiology·Robert P RyanJ Maxwell Dow
Jun 15, 2016·Annual Review of Phytopathology·Marie-Agnès JacquesChristian Vernière

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