Colonization of maize and rice plants by strain Bacillus megaterium C4

Current Microbiology
Xuming LiuSanfeng Chen

Abstract

Bacillus megaterium C4, a nitrogen-fixing bacterium, was marked with the gfp gene. Maize and rice seedlings were inoculated with the, GFP-labeled B. megaterium C4 and then grown in gnotobiotic condition. Observation by confocal laser scanning microscope showed that the GFP-labeled bacterial cells infected the maize roots through the cracks formed at the lateral root junctions and then penetrated into cortex, xylem, and pith, and that the bacteria migrated slowly from roots to stems and leaves. The bacteria were mainly located in the intercellular spaces, although a few bacterial cells were also present within the xylem vessels, root hair cells, epidermis, cortical parenchyma, and pith cells. In addition, microscopic observation also revealed clearly that the root tip in the zone of elongation and differentiation and the junction between the primary and the lateral roots were the two sites for the bacteria entry into rice root. Therefore, we conclude that this Gram-positive nitrogen-fixer has a colonization pattern similar to those of many Gram-negative diazotrophs, such as Azospirillun brasilense Yu62 and Azoarcus sp. As far as we know, this is the first detailed report of the colonization pattern for Gram-positive diazotrophic...Continue Reading

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Mei-Xia WangShu-Zhen Yan
Aug 28, 2010·The Journal of Microbiology·Changsong ZouDiqiu Yu
Jul 25, 2009·Environmental Microbiology·Isolde FrancisDanny Vereecke
Mar 14, 2008·FEMS Microbiology Ecology·Pilar Prieto, Jesús Mercado-Blanco
Oct 8, 2016·Frontiers in Plant Science·Medhin H Kifle, Mark D Laing
Jul 1, 2017·International Journal of Molecular Sciences·Yongbin LiSanfeng Chen
Jan 27, 2018·Molecular Plant-microbe Interactions : MPMI·Xingxing KangChanghong Liu
Mar 6, 2016·Applied and Environmental Microbiology·Omri M FinkelShimshon Belkin
Oct 11, 2020·FEMS Microbiology Ecology·Ricardo D SantanderElena G Biosca
Jul 16, 2021·Applied Microbiology and Biotechnology·Rebekka BiedendieckDieter Jahn

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