Colonization with and acquisition of uropathogenic Escherichia coli as revealed by polymerase chain reaction-based detection

The Journal of Infectious Diseases
J R JohnsonT A Russo

Abstract

The prevalence of colonization with uropathogenic Escherichia coli and their reservoirs and routes of acquisition are incompletely defined. To help clarify these issues, polymerase chain reaction (PCR)-based strain typing assays were used to evaluate the fecal and vaginal E. coli flora of 11 volunteers. PCR detected the virulence genes papG, aer, and cnf significantly more frequently in mixed intestinal samples than in the corresponding predominant strains, evidence that traditional methods are suboptimal for detecting colonization with uropathogens. For strain typing, repetitive-element PCR was as discriminating as pulsed-field gel electrophoresis and O:H serotyping but more convenient. Molecular epidemiologic analysis of subjects' E. coli suggested emergence of occult uropathogenic strains from within the host's own intestinal flora, strain sharing between household members, and de novo acquisition of (unshared) uropathogenic strains. These methods should facilitate the studies needed to clarify the relative contributions of these three pathways to the pathogenesis of urinary tract infection.

Citations

Dec 3, 2003·Current Infectious Disease Reports·Amee R. Manges
Aug 26, 2000·Journal of Microbiological Methods·J R Johnson
Jul 3, 1999·Trends in Microbiology·E V Sokurenko, D E Dykhuizen
Jun 17, 2006·Nature Reviews. Microbiology·Evgeni V SokurenkoDaniel E Dykhuizen
Aug 31, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James R Johnson, Parissa Delavari
Oct 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James R Johnson, Connie Clabots
Jul 22, 2008·The Journal of Antimicrobial Chemotherapy·Jesús Rodríguez-BañoAlvaro Pascual
May 7, 2003·Applied and Environmental Microbiology·Sandra L McLellanAlissa K Salmore
Nov 18, 2009·Infection and Immunity·Kelsey E Sivick, Harry L T Mobley
Feb 17, 2001·Infection and Immunity·J R JohnsonP Delavari
Oct 24, 2008·Journal of Clinical Microbiology·James R JohnsonEric Oswald
May 8, 2003·Journal of Clinical Microbiology·Sadjia BekalJosée Harel
Oct 2, 2008·Emerging Infectious Diseases·Amee R MangesPierre-Paul Tellier
Apr 29, 2016·Journal of Pathogens·Kesavaram PadmavathySikhamani Rajasekaran
Jun 17, 2009·Journal of Medical Microbiology·Khalifa Sifaw GhengheshJohn D Klena
Nov 20, 2012·Journal of Medical Microbiology·Kesavaram PadmavathySikhamani Rajasekaran
Feb 7, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B KrawczykJ Kur
Oct 22, 2005·International Journal of Medical Microbiology : IJMM·James R Johnson, Thomas A Russo
Aug 12, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Taous BachiriAbdelaziz Touati
Dec 12, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Taous BachiriAbdelaziz Touati
May 29, 2002·Environmental Microbiology·Stephen A Davis, David M Gordon
May 24, 2015·Applied and Environmental Microbiology·Michaela D J BlytonDavid M Gordon
Jun 18, 2002·Journal of Cellular and Molecular Medicine·C R UseinC Le Bouguénec
Nov 10, 2005·Journal of Medical Microbiology·Werner BokranzUte Römling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.