Colony formation and matrix production by human anulus cells: modulation in three-dimensional culture

Spine
H E GruberE N Hanley

Abstract

Human intervertebral disc cells from the anulus were tested in a study of colony formation and extracellular matrix (ECM) production during long-term three-dimensional culture with exposure to selected cytokines. Experimental studies were approved by the authors' Human Subjects Institutional Review Board. To quantitatively evaluate colony formation and qualitatively assess ECM production (using immunohistochemistry and in situ hybridization) in cells derived from Thompson Grades I to V discs and tested in culture with cytokines and nutrient supplementation. Human intervertebral disc cells offer special in vitro challenges because of the slow-growing nature of these cells and their need for specialized three-dimensional in vitro conditions, which permit the expression and production of proteoglycans and Type II collagen, two ECM products that are important for disc cell biology. Discs from 9 human subjects (2 control donors and 7 surgical patients, Thompson Grades I-V), mean age 35.8 years, were used to obtain anulus cells to be tested in three-dimensional agarose culture. Tests of specialized growth conditions included treatment with ITS (insulin-transferrin-sodium selenite supplement), insulin-like growth factor I (IGF-I), and...Continue Reading

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Citations

Jan 31, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Li JinXudong Li
Apr 17, 2007·The Spine Journal : Official Journal of the North American Spine Society·Helen E GruberEdward N Hanley
Dec 15, 2007·Biotechnology and Bioengineering·Gwendolyn M Hoben, Kyriacos A Athanasiou
Feb 28, 2007·Journal of Biomedical Materials Research. Part a·Xinxin Shao, Christopher J Hunter
Nov 8, 2006·The Spine Journal : Official Journal of the North American Spine Society·Kei MiyamotoHoward S An
Jul 14, 2016·Stem Cell Research & Therapy·Po-Hsin ChouOscar Kuang-Sheng Lee
Jun 9, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tomoko NakaiMasahiko Watanabe
Mar 13, 2014·Journal of Tissue Engineering and Regenerative Medicine·Shahriar SharifiRoel Kuijer
Nov 27, 2019·Spine Surgery and Related Research·Tadashi NukagaMasahiko Watanabe

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