Colony morphology variation of Burkholderia pseudomallei is associated with antigenic variation and O-polysaccharide modification

Infection and Immunity
Chanthiwa WikraiphatNarisara Chantratita

Abstract

Burkholderia pseudomallei is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS types. The predominant O-polysaccharide (OPS) is an unbranched heteropolymer with repeating d-glucose and 6-deoxy-l-talose residues in which the 6-deoxy-l-talose residues are variably replaced with O-acetyl and O-methyl modifications. We observed that primary clinical B. pseudomallei isolates with mucoid and nonmucoid colony morphologies from the same sample expressed different antigenic types distinguishable using an LPS-specific monoclonal antibody (MAb). MAb-reactive (nonmucoid) and nonreactive (mucoid) strains from the same patient exhibited identical LPS banding patterns by silver staining and indistinguishable genotypes. We hypothesized that LPS antigenic variation reflected modification of the OPS moieties. Mutagenesis of three genes involved in LPS synthesis was performed in B. pseudomallei K96243. Loss of MAb reactivity was observed in both wbiA (encoding a 2-O-acetyltransferase) and wbiD (putative methyl tr...Continue Reading

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Citations

Mar 25, 2016·Expert Review of Vaccines·Sophie A AschenbroichRobert J Hogan
Feb 26, 2016·Journal of Clinical Microbiology·Vichaya SuttisunhakulNarisara Chantratita
Mar 31, 2017·PLoS Neglected Tropical Diseases·Apinya PumpuangNarisara Chantratita
Oct 10, 2018·The American Journal of Tropical Medicine and Hygiene·Panjaporn ChaichanaSusanna J Dunachie
Jul 19, 2017·The American Journal of Tropical Medicine and Hygiene·Sineenart SengyeeNarisara Chantratita
Dec 29, 2020·Veterinary World·Vincentius Arca TestamentiJoko Pamungkas
Apr 4, 2021·Applied and Environmental Microbiology·Veerachat MuangsombutSunee Korbsrisate

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