Colorectal cancer cell-derived exosomes containing miR-10b regulate fibroblast cells via the PI3K/Akt pathway

Bulletin du cancer
Guangyao DaiJingcheng Zhang

Abstract

Cancer-associated fibroblasts (CAFs) contribute to the proliferation of colorectal cancer(CRC) cells. However, the mechanism by which CAFs develop in the tumor microenvironment remains unknown. Exosomes may be involved in activating CAFs. Using a miRNA expression profiling array, we determined the miRNA expression profile of secretory exosomes in CRC cells and then identified potential miRNAs with significant differential expression compared to normal cells via enrichment analysis. Predicted targets of candidate miRNAs were then assessed via bioinformatics analysis. Realtime qPCR, western blot, and cell cycle analyses were performed to evaluate the role of candidate exosomal miRNAs. Luciferase reporter assays were applied to confirm whether candidate exosomal miRNAs control target pathway expression. A CRC xenograft mouse model was constructed to evaluate tumor growth in vivo. Exosomes from CRC cells contained significantly higher levels of miR-10b than did exosomes from normal colorectal epithelial cells. Moreover, exosomes containing miR-10b were transferred to fibroblasts. Bioinformatics analysis identified PIK3CA, as a potential target of miR-10b. Luciferase reporter assays confirmed that miR-10b directly inhibited PIK3CA e...Continue Reading

Citations

Jan 15, 2019·Current Pharmaceutical Design·Reyhaneh Moradi-MarjanehSeyed H Aghaee-Bakhtiari
Nov 9, 2019·Epigenomics·Amir SavardashtakiMichael R Hamblin
Dec 1, 2019·Mutagenesis·Antonio FrancavillaAlessio Naccarati
Oct 17, 2019·IUBMB Life·Soheila MohammadiHamed Mirzaei
Nov 30, 2018·Journal of Cellular Physiology·Saeed NoorolyaiBehzad Baradaran
Jan 16, 2020·International Journal of Molecular Sciences·Maria Principia ScavoGianluigi Giannelli
May 10, 2020·Cells·Cristina P R XavierM Helena Vasconcelos
Jun 30, 2019·World Journal of Gastroenterology : WJG·Zhen-Hua YangGuang Ji
Nov 19, 2019·Journal of Oncology·Valentin VautrotJessica Gobbo
Feb 6, 2020·Cancers·Donatella LucchettiAlessandro Sgambato
Apr 18, 2020·Journal of Experimental & Clinical Cancer Research : CR·Shiming TanQianjin Liao
Sep 23, 2020·International Journal of Molecular Sciences·Issraa ShoucairCathie Garnis
Dec 5, 2019·International Journal of Molecular Sciences·Mercedes HerreraCristina Peña
Apr 8, 2020·Frontiers in Oncology·Fátima BaltazarSara Granja
Jan 14, 2021·Seminars in Cancer Biology·Alexander CocksMuller Fabbri
Jan 26, 2021·Cell Biochemistry and Function·Mahdi AhmadiJafar Rezaie
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Jenniffer LinaresAlexandre Calon
Dec 16, 2020·Cancers·Kevin Ho Wai YimRichard Chahwan
Feb 12, 2020·Cancer Letters·Yongwei XiaoTianyu Zhong
Feb 24, 2021·Journal of Cell Communication and Signaling·Baichao SunGuangdong Sun
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Qi ChenXiaolan Zhu
Jun 1, 2021·Molecular Biology Reports·Zeinab MoafianSeyed Isaac Hashemy
May 23, 2021·Human Immunology·Elham BaghbaniBehzad Baradaran
Jul 6, 2021·World Journal of Clinical Cases·Yvette UmwaliXin Zhang
Sep 9, 2020·Cancer Letters·Qingbin WuYong Peng

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.