Colorimetric and electrochemical detection of pathogens in water using silver ions as a unique probe.

Scientific Reports
Virendra KumarVijayender Bhalla

Abstract

The manuscript highlights the efficacy of silver ions to act as a unique probe for the detection of bacterial contamination in water samples. The bacterial cell membrane adherence property of the silver ions was employed to develop two different bacterial detection assays employing colorimetric and electrochemical techniques. In one of the schemes, silver ion was used directly as a detector of bacteria in a colorimetric assay format, and in the other scheme surface-functionalized antibodies were used as a primary capture for specific detection of Salmonella enterica serovar Typhi. The colorimetric detection is based on silver-induced inhibition of urease activity and silver ion utilization by bacteria for the rapid screening of enteric pathogens in water. The specific detection of bacteria uses an antibody-based electrochemical method that employs silver as an electrochemical probe. The ability of silver to act as an electrochemical probe was investigated by employing Anodic Stripping Voltammetry (ASV) for targeted detection of Salmonella Typhi. For further insights into the developed assays, inductively coupled plasma mass spectrometry (ICP-MS) and transmission electron microscopy (TEM) studies were performed. The sensitivity ...Continue Reading

References

Sep 1, 1991·Applied and Environmental Microbiology·L A BraunsJ D Oliver
Jun 1, 1974·Canadian Journal of Microbiology·P D Bragg, D J Rainnie
Mar 28, 2002·Journal of Enzyme Inhibition·C D Tormanen
Jun 19, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Wiesława ZaborskaZofia Olech
Feb 5, 2008·Applied and Environmental Microbiology·Woo Kyung JungYong Ho Park
Jun 30, 2010·Environmental Science & Technology·Georgios A Sotiriou, Sotiris E Pratsinis
Jul 7, 2012·Nano Letters·Zong-ming XiuPedro J J Alvarez
Dec 12, 2012·Chemistry Central Journal·Nana DuShuisheng Chen
Mar 2, 2013·Surgical Infections·Amani D PolitanoRobert G Sawyer
Mar 5, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wolfgang Maret
Jan 7, 2014·Analytical Chemistry·Haibo ZhouChristoph Haisch
Apr 24, 2015·Scientific Reports·Racheli Ben-Knaz WakshlakDavid Avnir
Sep 30, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yunfeng Lin, Ashton T Hamme
Jun 24, 2016·Expert Review of Molecular Diagnostics·Lars B LaurentiusMarc D Porter
Jan 21, 2017·Analytical Chemistry·Xiuli FuJaebum Choo
Apr 1, 2017·Journal of Nanobiotechnology·Teodora MocanLucian Mocan
Aug 2, 2018·Journal of Water and Health·Gerard N Stelma
Nov 24, 2018·The Analyst·P RajapakshaJ Chapman
Nov 25, 2018·Biosensors & Bioelectronics·Indra Memdi KhorisEnoch Y Park
Mar 19, 2019·Chemical Communications : Chem Comm·Pargat SinghVijayender Bhalla
Jan 3, 2020·ACS Applied Materials & Interfaces·T MokabberY T Pei

❮ Previous
Next ❯

Methods Mentioned

BETA
Surface-enhanced Raman scattering
enzyme assay

Software Mentioned

PlasmaLab
GraphPad Prism
Microsoft Office PowerPoint
IMTECH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.