Colorimetric and Fluorescent Bimodal Ratiometric Probes for pH Sensing of Living Cells

Chemistry, an Asian Journal
Yuan-Yuan LiuDe-Ming Kong

Abstract

pH measurement is widely used in many fields. Ratiometric pH sensing is an important way to improve the detection accuracy. Herein, five water-soluble cationic porphyrin derivatives were synthesized and their optical property changes with pH value were investigated. Their pH-dependent assembly/disassembly behaviors caused significant changes in both absorption and fluorescence spectra, thus making them promising bimodal ratiometric probes for both colorimetric and fluorescent pH sensing. Different substituent identity and position confer these probes with different sensitive pH-sensing ranges, and the substituent position gives a larger effect. By selecting different porphyrins, different signal intensity ratios and different fluorescence excitation wavelengths, sensitive pH sensing can be achieved in the range of 2.1-8.0. Having demonstrated the excellent reversibility, good accuracy and low cytotoxicity of the probes, they were successfully applied in pH sensing inside living cells.

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Citations

Mar 19, 2020·Chembiochem : a European Journal of Chemical Biology·Karolis NorvaišaMathias O Senge
Jul 4, 2020·Scientific Reports·Gaurav PandeyAbhijeet Joshi
Dec 11, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Rini MajumderPartha Pratim Parui
Feb 23, 2017·Chemical Reviews·Roberto PaolesseCorrado Di Natale

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