Colorimetric assay for biofilms in wet processing conditions.

Journal of Industrial Microbiology & Biotechnology
Judy W Arnold

Abstract

Controlling bacterial biofilms is necessary for food safety and industrial processing in clean room environments. Our goal was to develop a method to quantitatively measure biofilm produced by pathogens under wet poultry production and processing conditions. Stainless steel and glass coupons were incubated in aqueous media containing reduced nutrients and exposed to Listeria monocytogenes under static temperature and humidity conditions. Samples were measured separately by biofilm assay and viable cell density, and then confirmed by spectrophotometry and microscopy. The biofilm assay resulted in different t groupings from the cell density. The mean from the biofilm assay was 0.50, and the error% was 0.595. The mean of the log10 density (cfu/cm2) was 5.90, and the standard deviation ranged from 0.127 to 0.438 on 24 coupons. The typical sequence of biofilm development, followed by microscopy of biofilm grown on glass coupons, exhibited a change from dispersed single cells to an all-over pattern of clumps with few dispersed cells. L. monocytogenes formed biofilms on all of the substrata tested. Bacterial counts from planktonic cultures at 24, 48, 72, and 144 h confirmed that L. monocytogenes remained viable throughout the experime...Continue Reading

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Citations

Dec 30, 2009·Journal of Industrial Microbiology & Biotechnology·Fraddry D'SouzaPeter Willemsen
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Dec 21, 2017·International Journal of Environmental Research and Public Health·Tiziana PetrachiElena Veronesi
Aug 31, 2018·Scientific Reports·Jason TasseFrédéric Laurent

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