Colorimetric detection of DNA at the nanomolar level based on enzyme-induced gold nanoparticle de-aggregation

Mikrochimica Acta
Qingling LiuZhengbo Chen

Abstract

The authors describe a colorimetric method for the determination of DNA based on the deaggregation of gold nanoparticles (AuNPs) induced by exonuclease III (Exo III). DNA amplification is accomplished by Exo III to generate large quantities of the residual DNA. Residual DNA tethers onto the surfaces of AuNPs which prevents their aggregation. Hence, the color of the solution is red. However, in the absence of DNA, salt-induced aggregation is not prevented, and the bluish-purple color of the aggregated AuNPs is observed. The ratio of absorbances at 525 and 625 nm increases up to 150 nM DNA concentrations, and the LOD is as low as 3.0 nM. It is shown that the presence of 300 nM concentrations of random DNA (with a mass up to 10-fold that of target DNA) does not interfere. The method was successfully applied to the analysis of DNA in spiked serum samples. The method is simple, reliable, and does not require complicated amplification steps and expensive instrumentation. Graphical abstract Schematic of a sensing strategy for DNA detection by exonuclease III-induced deaggregation of gold nanoparticles. DNA concentrations as  low as 3 nM can be detected via colorimetric monitoring of the color change from red to purple-blue.

References

Jan 30, 2004·Experimental & Molecular Medicine·P O VardevanyanA T Karapetyan
Mar 12, 2011·Small·Jeroen PolletJeroen Lammertyn
Jul 23, 2013·Chemical Communications : Chem Comm·Fenglei GaoHuangxian Ju
May 16, 2015·Biosensors & Bioelectronics·Yun Kyung Jung, Hyun Gyu Park
Jun 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Li Yin ChauThomas M H Lee

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