Colorimetric detection of kanamycin based on analyte-protected silver nanoparticles and aptamer-selective sensing mechanism

Analytica Chimica Acta
Yuanyuan XuYuanshu Zhang

Abstract

In this work, a novel colorimetric detection method for kanamycin (Kana), a widely used aminoglycoside antibiotic, has been developed using unmodified silver nanoparticles (AgNPs) as sensing probe. The method is designed based on the finding that the analyte (Kana) can protect AgNPs against salt-induced aggregation, and nucleic acid aptamers can decrease the risk of false positives through an aptamer-selective sensing mechanism. By use of the proposed method, selective quantification of Kana can be achieved over the concentration range from 0.05 to 0.6 μg mL(-1) within 20 min. The detection limit is estimated to be 2.6 ng mL(-1), which is much lower than the allowed maximum residue limit. Further studies also demonstrate the applicability of the proposed method in milk samples, revealing that the method may possess enormous potential for practical detection of Kana in the future.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Apr 29, 2006·Journal of Veterinary Science·Yong JinMun-Han Lee
Jul 30, 2008·Analytical Chemistry·Hui WeiErkang Wang
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chang-Zhu YuWu-Er Gan
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban

Citations

Apr 18, 2016·Biosensors & Bioelectronics·Rezvan Yazdian RobatiSeyed Mohammad Taghdisi
Jun 20, 2016·Biosensors & Bioelectronics·Baris A BorsaV Cengiz Ozalp
Jul 23, 2016·Sensors·Tao YangJunwen Liu
Jun 13, 2018·Biosensors·Asol MehlhornYvonne Joseph
Oct 3, 2020·Analytical Methods : Advancing Methods and Applications·Xin YaoChangjun Hou
Jun 19, 2018·Frontiers in Chemistry·Qian SunAlexander F Routh
Oct 13, 2020·Critical Reviews in Analytical Chemistry·M Z H Khan
Jan 7, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fauzan AmriBrian Yuliarto

Related Concepts

Metazoa
Bacteriocidal Agents
Bos indicus
Colorimetry
Kantrex
Silver
Aptamers, Nucleotide
Metal Nanoparticles
Limit of Detection
Antibiotics, Aminoglycoside

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.