Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots

Mikrochimica Acta
Qian WangFengFu Fu

Abstract

A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2 is capable of oxidizing the substrate 3,3'5,5'-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 µM GSH concentration range, with a 0.095 μM detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries. Graphical abstract Schematic of the preparation of a nanohybrid composed of manganese dioxide and carbon based dots (MnO2/CDs). They can be used for the colorimetric detection of glutathione (GSH) based on the color change of 3,3'5,5'-tetramethylbenzidine (TMB).

References

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Citations

Dec 5, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mohamed Ibrahim HalawaGuobao Xu
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hemiao ZhaoCaixia Yin
Jun 10, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Liu ZhangBaoxin Li
Mar 17, 2021·The Analyst·Hafiz Muhammad JunaidMadeeha Batool
Mar 9, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Huiwen ZhangJuan Wang

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