Colorimetric Immunosensor by Aggregation of Photochemically Functionalized Gold Nanoparticles

ACS Omega
Marzia IarossiBartolomeo Della Ventura

Abstract

A colorimetric immunosensor based on local surface plasmon resonance by gold nanoparticles is presented, and its application for the detection of human immunoglobulin G (IgG) is demonstrated. The color change of the colloidal solution is produced by nanoparticle aggregation, a process that can be tuned by the presence of the analyte once the nanoparticles are functionalized. In comparison to common functionalization techniques, the procedure described here is simpler, low-cost, and effective in binding antibodies upright on the gold surface. The dose-response curve is similar to that resulting in typical immunoassay platforms and is satisfactorily described by the proposed theoretical model. Human IgG at concentration levels of few hundreds of nanograms per milliliter can be detected by eyes within a few minutes, thereby making the colorimetric immunosensor proposed here a powerful tool in several areas, with urine test in medical diagnostics being the most immediate.

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Citations

Dec 3, 2020·Nature Communications·Antonio MinopoliRaffaele Velotta
Mar 7, 2020·Photodiagnosis and Photodynamic Therapy·K AkshayaR Murugesan
Nov 6, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Ghazale FaridfarAfshin Samimi Nemati
Sep 30, 2020·ACS Sensors·Bartolomeo Della VenturaRaffaele Velotta
Jun 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jiajia YangJiping Chen
Jan 5, 2019·ACS Applied Materials & Interfaces·Bartolomeo Della VenturaRaffaele Velotta
Oct 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Weizheng WangSundaram Gunasekaran
Oct 12, 2021·Critical Reviews in Biotechnology·Chitra Padmakumari KurupMinhaz Uddin Ahmed

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Methods Mentioned

BETA
biosensors
surface plasmon resonance
surface plasmon coupling
quartz crystal microbalances
biosensor
dynamic light scattering

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