Colorimetric one-tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge.

Journal of Clinical Microbiology
M F ShaioJ Y Liu

Abstract

A colorimetric one-tube nested PCR was developed for the detection of Trichomonas vaginalis in clinical vaginal discharge specimens. A family of 650-bp specific DNA repeats from the T. vaginalis genome was targeted. There was no cross-reaction with human DNA or other infectious agents, including Pentatrichomonas hominis and Giardia lamblia. The colorimetric assay was applied as an adjunct to nested PCR for semiquantitative determination of T. vaginalis DNA at levels corresponding to 1 to 1,000 parasites. PCR of samples prepared by a rapid boiling method was as sensitive and specific as PCR of samples prepared by the standard DNA extraction method: the equivalent of one T. vaginalis organism in 20 microliters of vaginal discharge could be detected. The colorimetric nested PCR was compared with wet mount and culture for the detection of T. vaginalis. A total of 378 clinical vaginal discharge specimens from symptomatic patients were examined; 31 patients were positive for T. vaginalis both by culture and by nested PCR. However, only 17 of these 31 patients were positive by wet mount examination. In addition, of 113 asymptomatic patients, 9 were positive for T. vaginalis by nested PCR. Of these nine PCR-positive patients, only two ...Continue Reading

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Citations

Apr 20, 2002·Current Opinion in Infectious Diseases·Michael W. Lehker, John F. Alderete
May 24, 2003·Memórias do Instituto Oswaldo Cruz·Sheila B LeckeGeraldo A De Carli
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Jan 15, 2008·Current Opinion in Infectious Diseases·Victoria J Johnston, David C Mabey
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Jul 30, 2011·Diagnostic Microbiology and Infectious Disease·Claude Mambo MuvunyiElizaveta Padalko

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