Colorimetric PCR-Based microRNA Detection Method Based on Small Organic Dye and Single Enzyme

Analytical Chemistry
Juan DongZhuo Tang

Abstract

microRNAs (miRNAs) have been a class of promising disease diagnostic biomarkers and therapeutic targets for their important biological functions. However, because of the high homology, interference from precursors (pri-miRNA, pre-miRNA), as well as limitations in the current assay technologies, it poses high demand and challenge for a specific, efficient, and economic miRNA assay method. Here, we propose a new miRNA detection method based on a label-free probe and a small organic dye with sequence dependence, realizing the sequence-specific and colorimetric detection of target miRNA. What is pleasantly surprising, only one enzyme is enough to propel the whole miRNA assay process, greatly simplifying the reaction component and detection process. Together with PCR amplification for the high enough sensitivity and three checks for specificity control, a detection limit of 5 fM was obtained and even one mutation could be discriminated visually. Overall, the new method makes much progress in convenience and economy of PCR-based miRNA assay method so that miRNA assay is going to be more friendly and affordable.

References

Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Oct 21, 2006·Angewandte Chemie·Katharina B M Sauter, Andreas Marx
Jul 14, 2010·Biochimica Et Biophysica Acta·Fazli WahidYou Young Kim
Dec 6, 2012·Clinical Pharmacology and Therapeutics·S P Nana-Sinkam, C M Croce
Feb 21, 2013·Nature Communications·Yongyun ZhaoZhuo Tang
Oct 11, 2014·World Journal of Clinical Oncology·Jingyan XueZhao-Hui Wu

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Citations

May 10, 2019·Chemical Communications : Chem Comm·Jian LvRuo-Can Qian
Nov 11, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Qiaoling LiuXingwang Zheng
Dec 29, 2020·Journal of Pharmaceutical and Biomedical Analysis·Mingdi XuJunyang Zhuang
Dec 10, 2020·Science China. Chemistry·Yongxi ZhaoChunhai Fan
Dec 24, 2020·Analytical Methods : Advancing Methods and Applications·Yahui GuoHe Qian
Mar 12, 2020·ACS Synthetic Biology·Rashid AmanMagdy Mahfouz
May 24, 2019·ACS Applied Materials & Interfaces·Dan ZhuLianhui Wang
Aug 18, 2021·Analytica Chimica Acta·Moon Hyeok Choi, Young Jun Seo
Oct 8, 2019·Analytical Chemistry·Xian ChenHuanghao Yang

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