Column liquid chromatographic determination of clozapine and N-desmethylclozapine in human serum using solid-phase extraction

Journal of Chromatography. B, Biomedical Applications
R N Gupta

Abstract

A 0.5-ml aliquot of a serum sample, after the addition of a 50-microliters aliquot of a 5 microgram/ml solution of amoxapine as the internal standard, is vortex-mixed with 0.5 ml of acetonitrile and centrifuged. The supernatant is applied to a 1-ml BondElut C18 silica extraction column-conditioned with subsequent washings with 1 M HCL, methanol and water. After passing the sample at a slow rate, the column is washed twice with water and once with acetonitrile. The desired compounds are then eluted with a 0.25-ml aliquot of 35% perchloric acid-methanol (1:100, v/v). A 15 microliters aliquot of the eluate is injected onto a 150 x 4.6 mm I.D. column packed with 5-microns C8 silica particles and eluted at ambient temperature with a mobile phase of 0.1% tetramethylammonium perchlorate-acetonitrile (73:27, v/v) adjusted to pH 4.2 with 10% perchloric acid. The peaks are detected with an absorbance detector at 245 nm.

References

Apr 1, 1986·Biomedical Chromatography : BMC·Z R WangY L Zeng

❮ Previous
Next ❯

Citations

Mar 18, 2008·Biomedical Chromatography : BMC·Guodong ZhangMichael G Bartlett
Apr 8, 2009·Therapeutic Drug Monitoring·Wenzie NgRobert R Bies
Aug 1, 1997·Therapeutic Drug Monitoring·H WeigmannC Hiemke
Apr 11, 2018·Frontiers in Pharmacology·M Luisa ImazRocio Martin-Santos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here